{"title":"Phytochemical Screening, Phenolic Content, and Anti-Inflammatory Effect of <i>Foeniculum vulgare</i> Seed Extract.","authors":"Asma Cherbal, Mouna Bouabdallah, Mouna Benhalla, Saliha Hireche, Rachid Desdous","doi":"10.3746/pnf.2023.28.2.141","DOIUrl":null,"url":null,"abstract":"<p><p>Medicinal plants are promising sources of natural substances with biological functions and several drugs have been developed from traditional medicine. This study aimed to determine the chemical components of a hydromethanolic extract from <i>Foeniculum vulgare</i> seeds. Total phenolic, flavonoid, and flavonol contents were assessed, and gas chromatography-mass spectrometry (GC-MS) analysis was performed. To investigate the anti-inflammatory activity of <i>F. vulgare</i> seed hydromethanolic extract, its effects on protein denaturation, protease activity, membrane stabilization, and heat-induced hemolysis in red blood cells were evaluated <i>in vitro</i>. <i>F. vulgare</i> seed extract showed significant inhibition of protein denaturation (35.68±0.4%), protease activity (58.09±0.1%), and heat-induced hemolysis in red blood cells (9.67±0.3%) at concentrations of 200, 250, and 200 μg/mL, respectively, compared to the reference drug indomethacin (<i>P</i><0.001). This remarkable anti-inflammatory activity may be attributable to the abundance of flavonoids in the <i>F. vulgare</i> seed extract. GC-MS confirmed the presence of linalool and fatty acids (palmitic and oleic acids), which have potential anti-inflammatory activities. Therefore, the hydromethanolic extract of <i>F. vulgare</i> seeds may be a valuable anti-inflammatory candidate in the years ahead.</p>","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"28 2","pages":"141-148"},"PeriodicalIF":1.6000,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/68/44/pnfs-28-2-141.PMC10321444.pdf","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Preventive Nutrition and Food Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3746/pnf.2023.28.2.141","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 1
Abstract
Medicinal plants are promising sources of natural substances with biological functions and several drugs have been developed from traditional medicine. This study aimed to determine the chemical components of a hydromethanolic extract from Foeniculum vulgare seeds. Total phenolic, flavonoid, and flavonol contents were assessed, and gas chromatography-mass spectrometry (GC-MS) analysis was performed. To investigate the anti-inflammatory activity of F. vulgare seed hydromethanolic extract, its effects on protein denaturation, protease activity, membrane stabilization, and heat-induced hemolysis in red blood cells were evaluated in vitro. F. vulgare seed extract showed significant inhibition of protein denaturation (35.68±0.4%), protease activity (58.09±0.1%), and heat-induced hemolysis in red blood cells (9.67±0.3%) at concentrations of 200, 250, and 200 μg/mL, respectively, compared to the reference drug indomethacin (P<0.001). This remarkable anti-inflammatory activity may be attributable to the abundance of flavonoids in the F. vulgare seed extract. GC-MS confirmed the presence of linalool and fatty acids (palmitic and oleic acids), which have potential anti-inflammatory activities. Therefore, the hydromethanolic extract of F. vulgare seeds may be a valuable anti-inflammatory candidate in the years ahead.