Novel Selenium Peptides Obtained from Selenium-Enriched Cordyceps militaris Alleviate Neuroinflammation and Gut Microbiota Dysbacteriosis in LPS-Injured Mice

IF 6.2 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
Shujian Wu, Qingping Wu, Juan Wang, Yangfu Li, Bo Chen, Zhenjun Zhu, Rui Huang, Mengfei Chen, Aohuan Huang, Yizhen Xie, Chunwei Jiao, Yu Ding*
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引用次数: 16

Abstract

Increasing attention focuses on the relationship between neuroinflammation and Alzheimer’s disease (AD). The reports on the microbiota–gut–brain axis reveal that the regulation by gut microbiota is an effective way to intervene in neuroinflammation-related AD. In this study, two novel selenium peptides (Se-Ps), VPRKL(Se)M (Se-P1) and RYNA(Se)MNDYT (Se-P2), with neuroprotection effects were obtained from Se-enriched Cordyceps militaris. Se-P1 and Se-P2 pre-protection led to a 30 and 33% increase in the PC-12 cell viability compared to the damage group, respectively. Moreover, Se-Ps exhibited a significant pre-protection against LPS-induced inflammatory and oxidative stress in the colon and brain by inhibiting the production of pro-inflammatory mediators (p < 0.05) and malondialdehyde, as well as promoting anti-inflammatory cytokine level and antioxidant enzyme activity (p < 0.05), which may alleviate the cognitive impairment in LPS-injured mice (p < 0.05). Se-Ps not only repaired the intestinal mucosa damage of LPS-injured mice but also had a positive effect on gut microbiota dysbacteriosis by increasing the abundance of Lactobacillus and Alistipes and decreasing the abundance of Akkermansia and Bacteroides. Collectively, the antioxidant, anti-inflammatory, and regulating properties on gut microflora of Se-Ps contribute to their neuroprotection, supporting that Se-Ps could be a promising dietary supplement in the prevention and/or treatment of AD.

Abstract Image

从富硒蛹虫草中获得的新型硒肽可减轻lps损伤小鼠的神经炎症和肠道菌群失调
神经炎症与阿尔茨海默病(AD)之间的关系越来越受到关注。有关微生物-肠-脑轴的报道表明,肠道微生物群的调控是干预神经炎症相关性AD的有效途径。本研究从富硒蛹虫草中分离得到两种具有神经保护作用的新型硒肽(Se- ps) VPRKL(Se)M (Se- p1)和RYNA(Se)MNDYT (Se- p2)。与损伤组相比,Se-P1和Se-P2预保护分别使PC-12细胞活力提高了30%和33%。此外,Se-Ps通过抑制促炎介质的产生,对lps诱导的结肠和大脑炎症和氧化应激具有显著的预保护作用(p <0.05)和丙二醛,并促进抗炎细胞因子水平和抗氧化酶活性(p <0.05),可能减轻lps损伤小鼠的认知功能障碍(p <0.05)。Se-Ps不仅可以修复lps损伤小鼠的肠黏膜损伤,还可以通过增加Lactobacillus和Alistipes的丰度,降低Akkermansia和Bacteroides的丰度,对肠道菌群失调有积极的作用。总之,硒- ps的抗氧化、抗炎和调节肠道菌群的特性有助于其神经保护,支持硒- ps可能是一种有希望的预防和/或治疗AD的膳食补充剂。
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来源期刊
Journal of Agricultural and Food Chemistry
Journal of Agricultural and Food Chemistry 农林科学-农业综合
CiteScore
9.90
自引率
8.20%
发文量
1375
审稿时长
2.3 months
期刊介绍: The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.
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