Vaginal Infections during Pregnancy Increase Breast Milk Microbiome Alpha Diversity and Alter Taxonomic Composition.

IF 1.6 Q3 FOOD SCIENCE & TECHNOLOGY
Azka Ainun Nisaa, Chern-Ein Oon, Sasidharan Sreenivasan, Venugopal Balakrishnan, Deepa Rajendran, Jun-Jie Tan, Fatin Fazrina Roslan, Svetoslav Dimitrov Todorov, Woo-Sik Jeong, Feiyan Zhao, Nur Syafiqah Mohamad Nasir, Zakuan Zainy Deris, Heping Zhang, Yong-Ha Park, Guoxia Liu, Min-Tze Liong
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引用次数: 2

Abstract

We previously reported that breast milk from women with (W) or without (WO) vaginal yeast infection during pregnancy differs in its immunological and antimicrobial properties, especially against pathogenic vaginal Candida sp.. Here, we investigated the differences in microbiota profiles of breast milk from these groups. Seventy-two breast milk samples were collected from lactating mothers (W, n=37; WO, n=35). The DNA of bacteria was extracted from each breast milk sample for microbiota profiling by 16S rRNA gene sequencing. Breast milk from the W-group exhibited higher alpha diversity than that from the WO-group across different taxonomic levels of class (P=0.015), order (P=0.011), family (P=0.020), and genus (P=0.030). Compositional differences between groups as determined via beta diversity showed marginal differences at taxonomic levels of phylum (P=0.087), family (P=0.064), and genus (P=0.067). The W-group showed higher abundances of families Moraxellaceae (P=0.010) and Xanthomonadaceae (P=0.008), and their genera Acinetobacter (P=0.015), Enhydrobacter (P=0.015), and Stenotrophomonas (P=0.007). Meanwhile, the WO-group showed higher abundances of genus Staphylococcus (P=0.046) and species Streptococcus infantis (P=0.025). This study shows that, although breast milk composition is affected by vaginal infection during pregnancy, this may not pose a threat to infant growth and development.

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妊娠期阴道感染增加母乳微生物组α多样性并改变分类学组成。
我们以前报道过,怀孕期间阴道酵母菌感染(W)或未感染(WO)的妇女的母乳在免疫和抗菌特性方面存在差异,特别是对致病性阴道念珠菌。在这里,我们调查了这些组母乳中微生物群分布的差异。从哺乳期母亲中采集72份母乳样本(W, n=37;我们,n = 35)。从每个母乳样品中提取细菌DNA,通过16S rRNA基因测序进行微生物群分析。w组母乳在纲(P=0.015)、目(P=0.011)、科(P=0.020)和属(P=0.030)不同分类水平上的α多样性高于wo组。在门(P=0.087)、科(P=0.064)和属(P=0.067)的分类水平上,不同类群间的组成差异存在边际差异。w组Moraxellaceae (P=0.010)和Xanthomonadaceae (P=0.008)及其属Acinetobacter (P=0.015)、Enhydrobacter (P=0.015)和窄养单胞菌(P=0.007)的丰度较高。同时,wo组葡萄球菌属(P=0.046)和婴儿链球菌属(P=0.025)的丰度较高。这项研究表明,虽然母乳成分会受到怀孕期间阴道感染的影响,但这可能不会对婴儿的生长发育构成威胁。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Preventive Nutrition and Food Science
Preventive Nutrition and Food Science Agricultural and Biological Sciences-Food Science
CiteScore
3.40
自引率
0.00%
发文量
35
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