Protein vs. Energy Restriction Impact on Rat Testis' Function, Redox Status, and Histomorphometry.

IF 1.6 Q3 FOOD SCIENCE & TECHNOLOGY
Meddah Benabbou, Amine Allaoui, Aït Kaci Meriem Zahzeh, Ahmed Boualga, Touria Zahzeh
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Abstract

This study aimed to evaluate the effects of protein-restricted (PR) and energy restriction (ER) on male repro-ductive function. Eighteen weaning Wistar rats were divided into three groups, and were fed an experimental diet for five months. The first group, a control (C), received a diet containing 20% casein and 1.7×106 J/kg diet. The ER was given 50% less calories vs. C, while the PR group was given a low-protein diet (10% casein). The reproductive function was evaluated on serum and testis using anthropometrical, histological, hormonal, and oxidative parameters. The body weight was reduced by 37% and 40%, respectively, in the PR and ER groups vs. C. In the PR group, the testis relative weight was decreased, whereas that of the seminal vesicles was higher than that of C. The epididymis and prostate relative weights remained unchanged in the three experimental groups. Furthermore, serum testosterone concentrations were respectively 1.4- and 2.8-fold lower in the PR and ER groups vs. C, whereas no significant difference was found in luteinizing hormone and follicle-stimulating hormone levels among the groups. Compared with the C group, thiobarbituric acid reactive substance, carbonyl levels, glutathione, and glutathione reductase activity reduced significantly in PR, and specifically in ER rat's testis, whereas, catalase and superoxide dismutase activities increased. Furthermore, the testis and epididymis examination revealed histological alterations in the PR and ER groups. In conclusion, ER and PR diets could reduce the oxidant markers, even though they may alter the reproductive activity by probably altering testosterone production.

蛋白质和能量限制对大鼠睾丸功能、氧化还原状态和组织形态学的影响。
本研究旨在探讨限蛋白(PR)和限能(ER)对雄性生殖功能的影响。将18只断奶Wistar大鼠分为3组,连续5个月饲喂实验饲料。第一组为对照组(C),饲喂含20%酪蛋白和1.7×106 J/kg的饲粮。与C组相比,ER组摄入的卡路里少50%,而PR组摄入的是低蛋白饮食(10%酪蛋白)。用人体测量学、组织学、激素和氧化参数评价血清和睾丸的生殖功能。PR组和ER组体重分别比c组降低37%和40%。PR组睾丸相对重量降低,精囊相对重量高于c组。3个试验组的附睾和前列腺相对重量保持不变。此外,PR组和ER组的血清睾酮浓度分别比C组低1.4倍和2.8倍,而各组之间的促黄体生成素和促卵泡激素水平无显著差异。与C组相比,PR组,尤其是ER组大鼠睾丸中硫代巴比妥酸活性物质、羰基水平、谷胱甘肽和谷胱甘肽还原酶活性显著降低,过氧化氢酶和超氧化物歧化酶活性升高。此外,PR组和ER组的睾丸和附睾检查显示组织学改变。综上所述,ER和PR饮食可以降低氧化标记物,尽管它们可能通过改变睾丸激素的产生来改变生殖活动。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Preventive Nutrition and Food Science
Preventive Nutrition and Food Science Agricultural and Biological Sciences-Food Science
CiteScore
3.40
自引率
0.00%
发文量
35
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