Enhanced antioxidant and anti-inflammatory effects of bee pollen and honey hybrid formulation by hot-melt extrusion

IF 4.6 Q1 CHEMISTRY, APPLIED
Ha Yeon Lee , Suji Ryu , Han Sol You , Yoo Na Jeon , Minki Jin , Cheong-Weon Cho , Jong-Suep Baek
{"title":"Enhanced antioxidant and anti-inflammatory effects of bee pollen and honey hybrid formulation by hot-melt extrusion","authors":"Ha Yeon Lee ,&nbsp;Suji Ryu ,&nbsp;Han Sol You ,&nbsp;Yoo Na Jeon ,&nbsp;Minki Jin ,&nbsp;Cheong-Weon Cho ,&nbsp;Jong-Suep Baek","doi":"10.1016/j.fhfh.2023.100167","DOIUrl":null,"url":null,"abstract":"<div><p>Modifying the complex wall structure of bee pollen is a way to enhance the uptake of bioactive compounds. Bee Pollen and Bee Honey hybrid formulation (BPHF) are made by hot melt extrusion (HME), which is known to be the best cell wall structure-breaking method for active compound extraction because it can break the fibrous matrix of the compounds. Honey and bee pollen mixtures showed increased antioxidant activity with increased phenolic and flavonoid contents and decreased inhibitory concentration 50 (IC<sub>50</sub>) values compared to the control group. In addition, the content of gallic acid, one of the phenolic acids, increased about four times in the HME-F3 formulation compared to the control group, indicating that the phenol content was rich, but the HME-F3 formulation decreased in comparison. All samples had nano-sized particles. The Polydispersity index (PDI) value of the HME-F3 formulation was reduced and well distributed, and the stability of the particles was confirmed in the zeta potential results. Scanning electron microscopy (SEM) showed the image change of the sample after HME compared to the existing bee pollen image, and Transmission electron microscopy (TEM) showed that the particles of the mixed formulation were uniformly dispersed. Fourier-transform Infrared Analysis (FT-IR) results showed that there were structural changes in the mixture formulation compared to the control, and X-ray Diffraction Analysis (XRD) results showed that all samples were amorphous. It was found that the HME-F3 formulation could resist inflammation and protect the function of the intestinal epithelial wall. The mRNA levels of TJ-related proteins in the mixed formulation were reduced compared to LPS treatment and control.</p></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"4 ","pages":"Article 100167"},"PeriodicalIF":4.6000,"publicationDate":"2023-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667025923000511/pdfft?md5=d4c09320635394db841585b6afd6d7fd&pid=1-s2.0-S2667025923000511-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Hydrocolloids for Health","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667025923000511","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 0

Abstract

Modifying the complex wall structure of bee pollen is a way to enhance the uptake of bioactive compounds. Bee Pollen and Bee Honey hybrid formulation (BPHF) are made by hot melt extrusion (HME), which is known to be the best cell wall structure-breaking method for active compound extraction because it can break the fibrous matrix of the compounds. Honey and bee pollen mixtures showed increased antioxidant activity with increased phenolic and flavonoid contents and decreased inhibitory concentration 50 (IC50) values compared to the control group. In addition, the content of gallic acid, one of the phenolic acids, increased about four times in the HME-F3 formulation compared to the control group, indicating that the phenol content was rich, but the HME-F3 formulation decreased in comparison. All samples had nano-sized particles. The Polydispersity index (PDI) value of the HME-F3 formulation was reduced and well distributed, and the stability of the particles was confirmed in the zeta potential results. Scanning electron microscopy (SEM) showed the image change of the sample after HME compared to the existing bee pollen image, and Transmission electron microscopy (TEM) showed that the particles of the mixed formulation were uniformly dispersed. Fourier-transform Infrared Analysis (FT-IR) results showed that there were structural changes in the mixture formulation compared to the control, and X-ray Diffraction Analysis (XRD) results showed that all samples were amorphous. It was found that the HME-F3 formulation could resist inflammation and protect the function of the intestinal epithelial wall. The mRNA levels of TJ-related proteins in the mixed formulation were reduced compared to LPS treatment and control.

热熔挤压法增强蜂花粉与蜂蜜混合配方的抗氧化和抗炎作用
修改蜂花粉复杂的壁结构是提高生物活性化合物吸收的一种方法。蜂花粉与蜂蜂蜜混合配方(BPHF)采用热熔挤压法(HME)制备,该方法可破坏活性化合物的纤维基质,被认为是最佳的破坏活性化合物提取细胞壁结构的方法。与对照组相比,蜂蜜和蜂花粉混合物的抗氧化活性增加,酚类和类黄酮含量增加,抑制浓度50 (IC50)值降低。此外,HME-F3配方中酚酸之一没食子酸的含量比对照组增加了约4倍,说明酚含量丰富,但HME-F3配方的酚含量相对较低。所有样品都含有纳米级颗粒。HME-F3配方的多分散性指数(PDI)值降低且分布均匀,zeta电位结果证实了颗粒的稳定性。扫描电镜(SEM)显示了HME后样品与现有蜂花粉图像的图像变化,透射电镜(TEM)显示混合配方的颗粒均匀分散。傅里叶变换红外分析(FT-IR)结果表明,与对照组相比,混合物的配方发生了结构变化,x射线衍射分析(XRD)结果表明,所有样品都是无定形的。发现HME-F3制剂具有抗炎和保护肠上皮壁功能。与LPS处理和对照组相比,混合制剂中tj相关蛋白mRNA水平降低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
4.50
自引率
0.00%
发文量
0
审稿时长
61 days
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信