Can non-typeable Haemophilus influenzae carriage surveillance data infer antimicrobial resistance associated with otitis media?

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-02-07 eCollection Date: 2023-03-01 DOI:10.1002/ped4.12364

Sophia Bowman-Derrick, Tegan M Harris, Jemima Beissbarth, Mariana Kleinecke, Katrina Lawrence, Teresa M Wozniak, Amy Bleakley, Angela Rumaseb, Michael J Binks, Robyn L Marsh, Peter S Morris, Amanda J Leach, Heidi Smith-Vaughan

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Abstract

Importance: In remote communities of the Northern Territory, Australia, children experience high rates of otitis media (OM), commonly caused by non-typeable Haemophilus influenzae (NTHi). Few data exist on antibiotic susceptibility of NTHi from OM.

Objective: To determine whether population-level nasopharyngeal NTHi antibiotic susceptibility data could inform antibiotic treatment for OM.

Methods: NTHi isolates (n = 92) collected from ear discharge between 2003 and 2013 were selected to time- and age-match NTHi isolates from the nasopharyngeal carriage (n = 95). Antimicrobial susceptibility were tested. Phylogenomic trees and a genome-wide association study (GWAS) were performed to determine the similarity of nasopharyngeal and ear isolates at a population level.

Results: Among 174 NTHi isolates available for antimicrobial susceptibility testing, 10.3% (18/174) were resistant to ampicillin and 9.2% (16/174) were resistant to trimethoprim-sulfamethoxazole. Small numbers of isolates (≤3) were resistant to tetracycline, chloramphenicol, or amoxicillin-clavulanic acid. There was no statistical difference in the proportion of ampicillin-resistant (P = 0.11) or trimethoprim-sulfamethoxazole-resistant isolates (P = 0.70) between ear discharge and nasopharynx-derived NTHi isolates. Three multi-drug resistant NTHi isolates were identified. Phylogenomic trees showed no clustering of 187 Haemophilus influenzae isolates based on anatomical niche (nasopharynx or ear discharge), and no genetic variations that distinguished NTHi derived from ear discharge and nasopharyngeal carriage were evident in the GWAS.

Interpretation: In this population-level study, nasopharyngeal and ear discharge isolates did not represent distinct microbial populations. These results support tracking of population-level nasopharyngeal NTHi antibiotic resistance patterns to inform clinical management of OM in this population.

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非类型流感嗜血杆菌带菌监测数据能否推断出中耳炎的抗菌药耐药性？

重要性：在澳大利亚北领地的偏远社区，儿童中耳炎（OM）发病率很高，通常由非类型流感嗜血杆菌（NTHi）引起。关于中耳炎鼻嗜血杆菌对抗生素敏感性的数据很少：确定人群水平的鼻咽部 NTHi 抗生素敏感性数据能否为 OM 的抗生素治疗提供依据：2003年至2013年期间从耳道分泌物中采集的NTHi分离株（n = 92）与鼻咽带菌的NTHi分离株（n = 95）在时间和年龄上相匹配。测试了抗菌药敏感性。通过系统发生树和全基因组关联研究（GWAS）确定鼻咽和耳部分离物在群体水平上的相似性：结果：在可进行抗菌药敏感性测试的 174 个鼻咽癌病毒分离株中，10.3%（18/174）对氨苄西林耐药，9.2%（16/174）对三甲双胍-磺胺甲噁唑耐药。少数分离株（≤3 株）对四环素、氯霉素或阿莫西林-克拉维酸耐药。耳道分泌物和鼻咽源 NTHi 分离物中耐氨苄西林（P = 0.11）或耐三甲氧苄青霉素-磺胺甲噁唑（P = 0.70）的比例没有统计学差异。发现了三种耐多药的 NTHi 分离物。系统发生树显示，187 个流感嗜血杆菌分离物没有根据解剖位点（鼻咽或耳道）进行聚类，在基因组学分析中也没有发现可区分耳道和鼻咽携带的 NTHi 的基因变异：在这项人群研究中，鼻咽和耳道分泌物分离物并不代表不同的微生物种群。这些结果支持对人群水平的鼻咽NTHi抗生素耐药性模式进行追踪，以便为该人群的OM临床管理提供依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.