2S albumin g13 polypeptide, less related to Fag e 2, can be eliminated in common buckwheat (Fagopyrum esculentum Moench) seeds

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY
Fakhrul Islam Monshi , Tomoyuki Katsube-Tanaka
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引用次数: 1

Abstract

2S albumin (g11, g13, g14, and g28) is an important allergen in common buckwheat (Fagopyrum esculentum). g13 is hydrophobic, rare in seeds, and may show distinct allergenicity from the others; therefore, we tried to eliminate this protein. Phylogenetic and property distance analyses indicated g13 is less related to g14 (Fag e 2) than g11/g28 is related to g14, particularly in the second domain containing the II and III α-helices. A null allele with a 531 bp insertion in the coding region was found for g13 at an allele frequency of 2 % in natural populations of common buckwheat. The g13_null allele homozygote accumulated no g13 protein. A BLAST search for the 531 bp insertion suggested the insert-like sequence resided frequently in the buckwheat genome, including the self-incompatibility responsible gene ELF3 in Fagopyrum tataricum. The g13_null insert-like sequence could, therefore, help in producing hypoallergenic cultivars, and expand the genetic diversity of buckwheat.

在普通荞麦(Fagopyrum esculentum Moench)种子中可以消除与Fag e2不相关的2S白蛋白g13多肽
2S白蛋白(g11、g13、g14和g28)是普通荞麦(Fagopyrum esculentum)中重要的过敏原。G13是疏水的,在种子中罕见,可能表现出明显的过敏原性;因此,我们试图消除这种蛋白质。系统发育和性质距离分析表明,g13与g14的相关性低于g11/g28与g14的相关性,特别是在含有II和III α-螺旋的第二结构域。在普通荞麦自然居群中,以2%的等位基因频率发现了g13编码区插入531 bp的空等位基因。g13_null等位基因纯合子不积累g13蛋白。BLAST对531 bp插入序列的搜索表明,该插入样序列经常存在于荞麦基因组中,包括荞麦自交不亲和相关基因ELF3。因此,g13_null插入样序列可以帮助生产低过敏性品种,并扩大荞麦的遗传多样性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Food Chemistry Molecular Sciences
Food Chemistry Molecular Sciences Agricultural and Biological Sciences-Food Science
CiteScore
6.00
自引率
0.00%
发文量
83
审稿时长
82 days
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