Influence of structure and composition of diamond-like nanocomposite coatings on cell viability

IF 1.4 4区工程技术

Journal of Vacuum Science & Technology B Pub Date : 2021-10-14 DOI:10.1116/6.0001263

A. Grenadyorov, A. Solovyev, K. Oskomov, T. Santra, Pallavi Gupta, Dmitriy S. Korneev

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引用次数: 1

Abstract

This paper investigates the influence of the structure and properties of diamondlike nanocomposite (DLN or a-C:H:SiOx) coatings synthesized by plasma-assisted chemical vapor deposition on cell viability and coating biocompatibility. The structure and properties of the DLN coatings are changed by the negative pulse amplitude of the bipolar bias voltage of the substrate. The structure of the obtained DLN coatings is studied by Fourier-transform infrared spectroscopy and Raman spectroscopy. Atomic force microscopy provides angstrom-level surface-profiling information. The microhardness testing of the DLN coatings is performed on a nanohardness indenter of a three-sided Berkovich pyramid. It is shown that the higher roughness of the substrate surface, the growth in the crystalline graphite content in the coating, and Si—C bonds improve the DLN coating biocompatibility deposited at a −500 V bias voltage and the cell viability (>98% of HeLa cells), resulting in a lower cell death (1–2%). It is demonstrated that DLN coatings can be applied in biomedicine.

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类金刚石纳米复合涂层的结构和组成对细胞活力的影响

研究了等离子体辅助化学气相沉积法制备的类金刚石纳米复合材料(DLN或a-C:H:SiOx)涂层的结构和性能对细胞活力和涂层生物相容性的影响。衬底双极偏置电压的负脉冲幅度改变了DLN涂层的结构和性能。利用傅里叶变换红外光谱和拉曼光谱研究了所得DLN涂层的结构。原子力显微镜提供埃级表面轮廓信息。在三面伯氏金字塔的纳米硬度压头上进行了DLN涂层的显微硬度测试。结果表明，衬底表面粗糙度的提高、涂层中结晶石墨含量的增加以及Si-C键的增加提高了- 500 V偏置电压下沉积的DLN涂层的生物相容性和细胞存活率(>98%的HeLa细胞)，从而降低了细胞死亡率(1-2%)。结果表明，DLN涂层在生物医学领域具有广阔的应用前景。

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来源期刊

Journal of Vacuum Science & Technology B 工程技术-工程：电子与电气

自引率

14.30%

发文量

审稿时长

2.5 months

期刊介绍： Journal of Vacuum Science & Technology B emphasizes processing, measurement and phenomena associated with micrometer and nanometer structures and devices. Processing may include vacuum processing, plasma processing and microlithography among others, while measurement refers to a wide range of materials and device characterization methods for understanding the physics and chemistry of submicron and nanometer structures and devices.