{"title":"Removal of protein in RNA extraction of waste brewing yeast","authors":"Lt, strong gt, 王知力 lt, 郑飞云, 李崎, 顾国贤","doi":"10.3969/J.ISSN.0254-5071.2006.03.009","DOIUrl":null,"url":null,"abstract":"Three methods of removing proteins in RNA were discussed in this paper.The best way was to add 40 U neutral protease per gram dry yeast,and to compare the effects of protein removal to the loss of RNA.The extraction yield of this crude RNA product was 5.75% while the removal ratio of protein was 96.6%.","PeriodicalId":9837,"journal":{"name":"China Brewing","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"China Brewing","FirstCategoryId":"1087","ListUrlMain":"https://doi.org/10.3969/J.ISSN.0254-5071.2006.03.009","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Three methods of removing proteins in RNA were discussed in this paper.The best way was to add 40 U neutral protease per gram dry yeast,and to compare the effects of protein removal to the loss of RNA.The extraction yield of this crude RNA product was 5.75% while the removal ratio of protein was 96.6%.