Phytochemical, elemental, physico-chemical, HPTLC and anticancer investigations of Ceropegia spiralis Wight. Tuber extracts

The Pharma Innovation Journal Pub Date : 2021-01-01 DOI:10.22271/TPI.2021.V10.I3B.5918

Khaja peer Mulla, Chaithra Dandu, Vasavi Thirumalanadhuni, Shaheena Shaik, Iliyaz Ali Shaik, Yasodamma Nimmanapalli

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引用次数: 3

Abstract

Phytochemical constituents are responsible for medicinal activity of plant species. Hence in the present study Qualitative and quantitative phytochemical screening, Physicochemical, elemental analysis and anticancer activity of Ceropegia spiralis tuber extracts were carried out. Qualitative and quantitative phytochemical analysis of tuber aqueous extract confirm the presence of various secondary metabolites like saponins, triterpenoids, steroids, tannins, alkaloids, flavonoids and phenols. The results suggest that the phytochemical properties for curing various ailments and possess potential anticancer, antimicrobial and antioxidant activities leads to the isolation of new and novel compounds. Physicochemical studies reveals that the dry matter 96.23%; followed by Water soluble extractive 14.28 %. Elemental analysis reveals Nitrogen 3.4 % followed by Zinc 218.9%. Along with this, macro and micro elements which are essential for maintaining the animal body were also determined quantitatively. Anticancer activity of C. spiralis exhibited potential towards MDAMB-231 (human breast cancer) cell lines, shows 78.30% cell death with cell viability 21.70% at 100 µg/ml. The presence of various bioactive compounds confirms the application of C. spiralis against many ailments by the traditional practitioners.

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棘棘的植物化学、元素、理化、HPTLC及抗癌研究。块茎提取物

植物化学成分是植物药用活性的重要组成部分。为此，本研究对棘叶薯蓣提取物进行了定性和定量的植物化学筛选、理化、元素分析和抗癌活性研究。块茎水提物的定性和定量植物化学分析证实了多种次生代谢产物的存在，如皂苷、三萜、类固醇、单宁、生物碱、类黄酮和酚类。结果表明，其具有治疗多种疾病的植物化学性质，并具有潜在的抗癌、抗菌和抗氧化活性，从而导致了新化合物的分离。理化研究表明，干物质含量为96.23%;其次为水溶性提取物14.28%。元素分析显示氮含量为3.4%，锌含量为218.9%。同时定量测定了维持动物机体所必需的宏、微量元素。在100µg/ml浓度下，螺旋锥虫对MDAMB-231(人乳腺癌)细胞株显示出潜在的抗癌活性，细胞死亡率为78.30%，细胞存活率为21.70%。各种生物活性化合物的存在证实了传统从业者对许多疾病螺旋体的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Pharma Innovation Journal

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