Sensitive and Colorimetric Detection of the Structural Evolution of Superoxide Dismutase with Gold Nanoparticles

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2009-01-26 DOI:10.1021/ac802099c

Surin Hong, Inhee Choi, Suseung Lee, Young In Yang, Taewook Kang, Jongheop Yi*

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引用次数: 74

Abstract

The detection and characterization of protein aggregates are critical in terms of advanced diagnostic applications and investigations of protein stability. A variety of analytical methods (e.g., circular dichroism, size exclusion chromatography, and fluorescence microscopy) have been used in this regard, but they are limited in the trace detection of the structural evolution of protein aggregation. Here we report the gold nanoparticle (AuNP)-based highly sensitive and colorimetric detection of the temporal evolution of superoxide dismutase (SOD1) aggregates implicated in the pathology of amyotrophic lateral sclerosis (ALS). For the temporal discrimination of SOD1 aggregation, AuNPs were conjugated with SOD1 monomers (SOD1?AuNPs). Upon exposure of the probes (SOD1?AuNPs) with SOD1 aggregates, significant changes in both surface plasmon resonance spectra and concomitant colors were observed which are attributed to the formation of probe aggregates of variable sizes onto the SOD1 aggregates.

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金纳米颗粒对超氧化物歧化酶结构演变的灵敏比色检测

蛋白质聚集体的检测和表征在先进的诊断应用和蛋白质稳定性研究方面至关重要。在这方面已经使用了各种分析方法(如圆二色、尺寸排除色谱和荧光显微镜)，但它们在蛋白质聚集结构演变的痕量检测方面受到限制。在这里，我们报告了基于金纳米颗粒(AuNP)的高灵敏度和比色法检测与肌萎缩性侧索硬化症(ALS)病理相关的超氧化物歧化酶(SOD1)聚集物的时间演变。为了区分SOD1聚集的时间，我们将AuNPs与SOD1单体偶联(SOD1?AuNPs)。当探针(SOD1?AuNPs)与SOD1聚集体暴露时，观察到表面等离子体共振光谱和伴随颜色的显着变化，这是由于在SOD1聚集体上形成了不同大小的探针聚集体。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.