Investigating the biological activities and prebiotic potential of exopolysaccharides Produced by Lactobacillus delbrueckii and Lacticaseibacillus rhamnosus: Implications for gut microbiota modulation and rheological properties in fermented milk

IF 4.6 Q1 CHEMISTRY, APPLIED
Mohammad Tarique , Abdelmoneim H. Ali , Jaleel Kizhakkayil , Ren-You Gan , Shao-Quan Liu , Afaf Kamal-Eldin , Mutamed Ayyash
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引用次数: 0

Abstract

The objective of this research was to explore the functional qualities of two EPSs produced by recently discovered LAB (Lactobacillus delbrueckii (EPS-LB3) and Lacticaseibacillus rhamnosus (EPS-MLB3)) that have potential probiotic benefits. The study involved evaluating their biological characteristics, such as their antioxidant, antidiabetic, antimicrobial, antibiofilm, and antiproliferative activities at various concentrations, as well as investigating their effects on the gut microbiome through in vitro fecal fermentation. Moreover, the study analyzed the rheological properties of the EPSs in fermented bovine milk. The average molecular weights of the extracted EPS were 3762.43 kDa and 1272.19 kDa with monosaccharide compositions of Glu:Rib:Man:Xyl (1.0:16.4:6.6:6.5) and Rib:Man:Xyl:GA:Ara (7.1:1.6:4.8:1.0:9.0) for EPS-LB3 and EPS-MLB3, respectively. EPS-LB3 and EPS-MLB3 at 250 mg/L showed scavenging rates of 34.0 ± 1.7 % and 37.5 ± 1.1 % for DPPH, 47.3 ± 0.8 % and 56.6 ± 0.7 % for ABTS, 38.3 ± 0.5 % and 43.5 ± 0.6 % for SD, 53.9 ± 0.1 % and 54.7 ± 0.1 % for SAS, 10.6 ± 0.1 % and 10.7 ± 0.2 % for HP, 88.8 ± 0.1 % and 84.8 ± 0.5 % for HRS, 80.0 ± 1.4 % and 84.5 ± 0.8 % for MC, as well as 60.6 ± 1.7 % and 58.1 ± 0.9 % for Lipid Oxidation, respectively, suggesting good antioxidant properties. They also exhibited antimicrobial and anti-biofilm effects against several foodborne pathogens, and antiproliferative activities against cancer cell lines. Additionally, the utilization of EPS by several probiotics indicated the prebiotic nature of EPS. The effect of both EPS on gut microbiome by fecal fermentation revealed that these EPS promoted selective bacteria like Faecalibacterium prausnitzii and Ruminococcus bromii in the gut, which are responsible for carbohydrate metabolism and short-chain fatty acid production.

研究德布吕氏乳杆菌和鼠李糖乳杆菌产生的胞外多糖的生物活性和益生元潜力:对发酵乳中肠道菌群调节和流变特性的影响
本研究的目的是探索最近发现的具有潜在益生菌益处的乳酸杆菌(德氏乳杆菌(EPS-LB3)和鼠李糖乳酸杆菌(EPS-MLB3))生产的两种EPS的功能品质。这项研究包括评估它们的生物学特性,如在不同浓度下的抗氧化、抗糖尿病、抗菌、抗生物膜和抗增殖活性,以及通过体外粪便发酵研究它们对肠道微生物组的影响。此外,本研究还分析了发酵牛乳中EPSs的流变特性。提取的EPS的平均分子量分别为3762.43kDa和1272.19kDa,EPS-LB3和EPS-MLB3的单糖组成分别为Glu:Rib:Man:Xyl(1.0:16.4:6.6:6.5)和Rib:Man-Xyl:GA:Ara(7.1:1.6:4.8:1.0:9.0)。EPS-LB3和EPS-MLB3在250mg/L时对DPPH的清除率分别为34.0±1.7%和37.5±1.1%,对ABTS的清除率为47.3±0.8%和56.6±0.7%,对SD的清除率依次为38.3±0.5%和43.5±0.6%,对SAS的清除率次之为53.9±0.1%和54.7±0.1%,对HP的清除率次于10.6±0.1%和10.7±0.2%,对HRS的清除率是88.8±0.1%和84.8±0.5%,对MC的清除率则分别为80.0±1.4%和84.5±0.8%,脂质氧化分别为60.6±1.7%和58.1±0.9%,表明其具有良好的抗氧化性能。它们还表现出对几种食源性病原体的抗菌和抗生物膜作用,以及对癌症细胞系的抗增殖活性。此外,几种益生菌对EPS的利用表明了EPS的益生元性质。两种EPS通过粪便发酵对肠道微生物组的影响表明,这些EPS促进了肠道中的选择性细菌,如普氏粪杆菌和溴瘤胃球菌,它们负责碳水化合物代谢和短链脂肪酸的产生。
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CiteScore
4.50
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