Implementation of wedged-serial protein crystallography at PROXIMA-1.

IF 2.4 3区 物理与天体物理 Q2 INSTRUMENTS & INSTRUMENTATION
Journal of Synchrotron Radiation Pub Date : 2022-03-01 Epub Date: 2022-01-17 DOI:10.1107/S1600577521013242
Igor Chaussavoine, Tatiana Isabet, Robin Lener, Pierre Montaville, Ramakrishna Vasireddi, Leonard M G Chavas
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引用次数: 0

Abstract

An approach for serial crystallography experiments based on wedged-data collection is described. This is an alternative method for recording in situ X-ray diffraction data on crystalline samples efficiently loaded in an X-ray compatible microfluidic chip. Proper handling of the microfluidic chip places crystalline samples at geometrically known positions with respect to the focused X-ray interaction area for serial data collection of small wedges. The integration of this strategy takes advantage of the greatly modular sample environment available on the endstation, which allows access to both in situ and more classical cryo-crystallography with minimum time loss. The method represents another optional data collection approach that adds up to the already large set of methods made available to users. Coupled with the advances in processing serial crystallography data, the wedged-data collection strategy proves highly efficient in minimizing the amount of required sample crystals for recording a complete dataset. From the advances in microfluidic technology presented here, high-throughput room-temperature crystallography experiments may become routine and should be easily extended to industrial use.

楔形序列蛋白晶体学在PROXIMA-1上的实现
在PROXIMA-1上实现基于微流体的楔形序列蛋白质晶体学为低样品消耗的原位数据收集实验开辟了新的可能性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
5.10
自引率
12.00%
发文量
289
审稿时长
4-8 weeks
期刊介绍: Synchrotron radiation research is rapidly expanding with many new sources of radiation being created globally. Synchrotron radiation plays a leading role in pure science and in emerging technologies. The Journal of Synchrotron Radiation provides comprehensive coverage of the entire field of synchrotron radiation and free-electron laser research including instrumentation, theory, computing and scientific applications in areas such as biology, nanoscience and materials science. Rapid publication ensures an up-to-date information resource for scientists and engineers in the field.
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