Targeting the Phosphatidylinositol 3-Kinase/AKT1 Signaling Pathway: Iphigenia indica Extracts Induce Apoptosis in Lung Adenocarcinoma Cells

Abstract

This study aimed to investigate the inhibitory effects of Iphigenia indica extracts on lung adenocarcinoma A549 cells through the PI3K/AKT1 signaling pathway. The researchers treated A549 cells with different concentrations of Iphigenia indica extracts and conducted various assays. The results showed that the viability of A549 cells decreased with increasing concentration of Iphigenia indica extracts. Iphigenia indica extracts and PI3K/AKT inhibitor had a higher inhibitory rate of cell proliferation and colony formation, reduced migration and invasion, and induced apoptosis in A549 cells compared to the control group. Furthermore, Iphigenia indica extracts and PI3K/AKT inhibitor reduced the protein levels of Bcl-2, PI3K, and AKT1 and increased the level of Bax. The findings suggest that Iphigenia indica extracts may inhibit malignant biological behaviors of lung adenocarcinoma cells through the PI3K/AKT1 signaling pathway by inducing apoptosis, inhibiting proliferation, migration and invasion, and regulating the expression of Bcl-2, Bax, PI3K, and AKT1. Overall, Iphigenia indica extracts may have potential as a therapeutic agent for lung adenocarcinoma.