The contribution of epiphytic microbiota in oat and Italian ryegrass to silage fermentation products and bacterial community structure of whole-crop maize

IF 5.2 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Chemical and Biological Technologies in Agriculture Pub Date : 2023-07-19 DOI:10.1186/s40538-023-00439-y

Siran Wang, Shiwei Hu, Zhihao Dong, Junfeng Li, Jie Zhao, Mudasir Nazar, Niaz Ali Kaka, Tao Shao

{"title":"The contribution of epiphytic microbiota in oat and Italian ryegrass to silage fermentation products and bacterial community structure of whole-crop maize","authors":"Siran Wang, Shiwei Hu, Zhihao Dong, Junfeng Li, Jie Zhao, Mudasir Nazar, Niaz Ali Kaka, Tao Shao","doi":"10.1186/s40538-023-00439-y","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>The purpose of this research is to study the contribution of epiphytic microbiota in fresh oat (OT), Italian ryegrass (IR) and whole-crop maize (MZ) to silage fermentation products and bacterial community structure of MZ. After γ-ray irradiation, the sterile MZ was treated via microbiota transplantation method: (1) sterile deionized water (STMZ); (2) microbiota epiphytic on MZ (MZMZ); (3) microbiota epiphytic on OT (MZOT); (4) microbiota epiphytic on IR (MZIR). Triplicate silos of each treatment were tested after 1, 3, 7, 15, 30 and 60 days of ensiling.</p><h3>Results</h3><p>MZMZ had higher (<i>P</i> < 0.05) lactic acid contents, and lower (<i>P</i> < 0.05) ammonia nitrogen and ethanol contents than MZIR and MZOT on day 60. The relative abundance of <i>Lactobacillus</i> in MZMZ decreased from 84.0% on day 3 to 44.7% on day 60. MZMZ had higher (<i>P</i> < 0.05) abundances of ‘Nucleotide metabolism’, ‘Replication and repair’ and ‘Membrane transport’, and lower (<i>P</i> < 0.05) abundance of ‘Amino acid metabolism’ than MZOT and MZIR on day 3.</p><h3>Conclusions</h3><p>The silage fermentation products of MZ were highly affected by the activity and compositions of epiphytic microbiota. The Enterobacteriaceae, <i>Hafnia-Obesumbacterium</i>, hetero-fermentative and acid-resistant <i>Lactobacillus</i> took primary responsibility for the high dry matter loss and ethanol contents and low lactic acid contents in MZ silage.</p><h3>Graphical Abstract</h3>\n <figure><div><div><div><picture><source><img></source></picture></div></div></div></figure>\n </div>","PeriodicalId":512,"journal":{"name":"Chemical and Biological Technologies in Agriculture","volume":"10 1","pages":""},"PeriodicalIF":5.2000,"publicationDate":"2023-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-023-00439-y","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical and Biological Technologies in Agriculture","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1186/s40538-023-00439-y","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

Background

The purpose of this research is to study the contribution of epiphytic microbiota in fresh oat (OT), Italian ryegrass (IR) and whole-crop maize (MZ) to silage fermentation products and bacterial community structure of MZ. After γ-ray irradiation, the sterile MZ was treated via microbiota transplantation method: (1) sterile deionized water (STMZ); (2) microbiota epiphytic on MZ (MZMZ); (3) microbiota epiphytic on OT (MZOT); (4) microbiota epiphytic on IR (MZIR). Triplicate silos of each treatment were tested after 1, 3, 7, 15, 30 and 60 days of ensiling.

Results

MZMZ had higher (P < 0.05) lactic acid contents, and lower (P < 0.05) ammonia nitrogen and ethanol contents than MZIR and MZOT on day 60. The relative abundance of Lactobacillus in MZMZ decreased from 84.0% on day 3 to 44.7% on day 60. MZMZ had higher (P < 0.05) abundances of ‘Nucleotide metabolism’, ‘Replication and repair’ and ‘Membrane transport’, and lower (P < 0.05) abundance of ‘Amino acid metabolism’ than MZOT and MZIR on day 3.

Conclusions

The silage fermentation products of MZ were highly affected by the activity and compositions of epiphytic microbiota. The Enterobacteriaceae, Hafnia-Obesumbacterium, hetero-fermentative and acid-resistant Lactobacillus took primary responsibility for the high dry matter loss and ethanol contents and low lactic acid contents in MZ silage.

Graphical Abstract

查看原文本刊更多论文

燕麦和意大利黑麦草附生菌群对玉米青贮发酵产物和细菌群落结构的贡献

本研究旨在研究鲜燕麦(OT)、意大利黑麦草(IR)和全玉米(MZ)中附生菌群对青贮发酵产物和MZ细菌群落结构的贡献。经γ射线照射后，采用菌群移植法处理无菌MZ:(1)无菌去离子水(STMZ);(2) MZ上的附生菌群(MZMZ);(3) OT上的附生菌群(MZOT);(4)红外附生菌群(MZIR)。分别在青贮1、3、7、15、30和60 d后对每个处理的3个重复筒仓进行试验。结果在第60天，与MZIR和MZOT相比，smzmz乳酸含量较高(P < 0.05)，氨氮和乙醇含量较低(P < 0.05)。乳酸菌的相对丰度从第3天的84.0%下降到第60天的44.7%。在第3天，MZMZ的“核苷酸代谢”、“复制与修复”和“膜运输”丰度高于MZOT和MZIR (P < 0.05)，而“氨基酸代谢”丰度低于MZOT和MZIR (P < 0.05)。结论MZ青贮发酵产物受附生菌群活性和组成的影响较大。MZ青贮干物质损失高、乙醇含量高、乳酸含量低的主要原因是肠杆菌科、Hafnia-Obesumbacterium、异发酵耐酸乳酸杆菌。图形抽象

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

6.80

自引率

3.00%

发文量

审稿时长

15 weeks

期刊介绍： Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.