Transcriptome and Micro-CT analysis unravels the cuticle modification in phosphine-resistant stored grain insect pest, Tribolium castaneum (Herbst)

IF 5.2 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Chemical and Biological Technologies in Agriculture Pub Date : 2023-09-08 DOI:10.1186/s40538-023-00466-9

Donghyeon Kim, Kyeongnam Kim, Yong Ho Lee, Sung-Eun Lee

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引用次数: 1

Abstract

Background

Phosphine (PH₃) resistance in stored grain insect pests poses a significant challenge to effective pest control strategies worldwide. This study delved into understanding PH₃-resistant mechanism, with the objective of informing robust and sustainable pest management strategies that could mitigate the impacts of PH₃ resistance.

Results

In this regard, the transcriptomic analysis identified 23 genes associated with chitin synthesis and cuticle formation, which showed significant expression in PH₃-resistant (R) strains compared to susceptible strains. Micro-computed tomography (Micro-CT) revealed an extended and tighter cuticular structure in the PH₃-R Tribolium castaneum than PH₃-susceptible strains but with no changes in the cuticle thickness. This altered cuticle structure may reduce PH₃ penetration through cuticles rather than completely closing spiracles during fumigation. It is also hypothesized to prevent water loss from the insect body, as water production decreased in PH₃-R T. castaneum due to the down-regulation of the electron transport chain function. Validation of several chitin synthesis gene expression levels revealed consistent results with those of transcriptomic analysis.

Conclusion

Overall, integrating physical treatments using synthetic amorphous silicates, water absorbents, and cuticle-damaging materials during PH₃ fumigation is recommended for its prolonged and controlled usage in the field.

Graphical abstract

查看原文本刊更多论文

转录组和Micro-CT分析揭示了耐磷化氢储粮害虫谷草的角质层修饰

储粮害虫对磷化氢(PH3)的抗性对世界范围内有效的害虫防治策略提出了重大挑战。本研究深入了解了PH3抗性机制，目的是为减轻PH3抗性影响的强大和可持续的有害生物管理策略提供信息。结果转录组学分析鉴定出23个与几丁质合成和角质层形成相关的基因，这些基因在ph3抗性菌株中表达显著高于敏感菌株。微计算机断层扫描(Micro-CT)显示，与ph3敏感菌株相比，PH3-R Tribolium castaneum的角质层结构更大、更紧，但角质层厚度没有变化。这种改变的角质层结构可能会减少PH3通过角质层的渗透，而不是在熏蒸过程中完全关闭气门。由于电子传递链功能的下调，PH3-R T. castaneum的产水量减少，因此也被认为可以防止昆虫体内的水分流失。几个几丁质合成基因表达水平的验证结果与转录组学分析结果一致。结论在PH3熏蒸过程中，建议采用合成无定形硅酸盐、吸水剂和破坏角质层材料进行综合物理处理，以实现PH3熏蒸在野外的长期控制使用。图形抽象

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

6.80

自引率

3.00%

发文量

审稿时长

15 weeks

期刊介绍： Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.