Effects of Exopolysaccharides from Lactiplantibacillus plantarum JLAU103 on Intestinal Immune Response, Oxidative Stress, and Microbial Communities in Cyclophosphamide-Induced Immunosuppressed Mice

IF 6.2 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
Ji Wang, Meihe Li, Yawen Gao, Hongmei Li, Li Fang, Chunlei Liu, Xiaoting Liu, Weihong Min*
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引用次数: 19

Abstract

This study investigated the effects of the exopolysaccharide from Lactiplantibacillus plantarum JLAU103 (EPS103) on the intestinal immune response, oxidative stress, intestinal mucosal barrier, and microbial community in cyclophosphamide-induced immune-suppressed mice. The results showed that EPS103 promoted the secretion of cytokines and the generation of secretory immunoglobulin A and mucin-2 in the small intestine of mice, which might be related to the activation of the MAPK pathway. Additionally, EPS103 protected against oxidative stress by activating antioxidation enzymes and Nrf2/Keap1 pathways. It also improved the intestinal physical barrier functions via regulating the ratio of villous height to crypt depth and upregulating the expression of tight-junction proteins. Meanwhile, EPS103 promoted the generation of short-chain fatty acids (SCFAs) and modulated the constituents of gut microbiota. These results suggested that EPS103 may modulate the intestinal immunoresponse relying on the regulation of SCFA production and gut microbiota in immunosuppressed mice, resulting in the activation of systemic immunity.

Abstract Image

植物乳杆菌JLAU103胞外多糖对环磷酰胺诱导免疫抑制小鼠肠道免疫应答、氧化应激和微生物群落的影响
本实验研究了植物乳杆菌JLAU103 (EPS103)胞外多糖对环磷酰胺诱导免疫抑制小鼠肠道免疫应答、氧化应激、肠黏膜屏障和微生物群落的影响。结果表明,EPS103促进小鼠小肠细胞因子的分泌,分泌性免疫球蛋白A和黏液蛋白2的产生,可能与MAPK通路的激活有关。此外,EPS103通过激活抗氧化酶和Nrf2/Keap1通路来保护氧化应激。它还通过调节绒毛高度与隐窝深度之比和上调紧密连接蛋白的表达来改善肠道物理屏障功能。同时,EPS103促进短链脂肪酸(SCFAs)的生成,调节肠道菌群组成。这些结果表明,EPS103可能通过调节免疫抑制小鼠的SCFA生成和肠道微生物群来调节肠道免疫反应,从而激活全身免疫。
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来源期刊
Journal of Agricultural and Food Chemistry
Journal of Agricultural and Food Chemistry 农林科学-农业综合
CiteScore
9.90
自引率
8.20%
发文量
1375
审稿时长
2.3 months
期刊介绍: The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.
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