Insight into biosorption of hexavalent chromium using isolated species Aspergillus Proliferans LA: A systemic and In silico studies

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Abhimati Shukla, L. Singh
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引用次数: 0

Abstract

Background: The wastewater disposal into the water bodies without removing the toxic heavy metals and other industrial impurities is a major issue these days. These heavy metals cause serious health issues to the human and animal life and also harm the environment and reduce the productivity of crops. A potent microorganism resistant to hexavalent chromium was isolated. The activity of this isolated strain was analyzed using in silico studies. Methods: In this study, a chromium-resistant fungus was isolated from the soil of the dumping sites of the tanneries in Kanpur, UP, India, followed by isolation by serial dilution, plating method, and finally, genome sequencing. It was identified as Aspergillus proliferans LA that is submitted to the National Collection for Industrial Microorganisms (NCIM) database with accession no. NCIM-1473. In the current study, the comparative analysis of the protein sequence of A. proliferans (NCIM-1473) was done against the known 53 protein sequences of the fungus and bacterial strains already reported for their chromium-resistant nature. The physical and chemical parameters of the known and isolated chromium-resistant proteins were analyzed using the ProtParam tool. The comparative study on the sequence and structural alignment of known and isolated chromium-resistant protein was done using EMBOSS-NEEDLE and FATCAT, respectively. Results: In this analysis, the top 10 strains showing similarity with A. proliferans (NCIM-1473) were reported and among which ChrI, chromium regulatory protein Cupriavidus metallidurans CH34 was showing maximum similarity with isolated chromium resistant protein for all the analysis, namely ProtParam, sequence, and FATCAT analysis. This strain, Cupriavidus metallidurans CH34, has been reported resistant against eight heavy metals, one of which is chromate, and was first identified in the heavy-metal contaminated sludge in a settling tank of Belgium. Conclusion: These studies conclude that the strain isolated in our laboratory (accession no. NCIM-1473) is potentially chromium resistant and a unique strain.
利用分离种增殖曲霉LA对六价铬的生物吸附的洞察:系统和计算机研究
背景:污水排入水体而不去除有毒重金属和其他工业杂质是目前的一个主要问题。这些重金属对人类和动物的生命造成严重的健康问题,也危害环境并降低作物的生产力。分离出一种抗六价铬的强效微生物。该分离菌株的活性用硅片研究进行了分析。方法:从印度北方邦坎普尔制革厂倾倒场土壤中分离出一株耐铬真菌,采用连续稀释、电镀等方法进行分离,最后进行基因组测序。经鉴定为增殖曲霉LA,已提交美国国家工业微生物收集(NCIM)数据库,登录号为:ncim - 1473。本研究将A. proliferans (NCIM-1473)的蛋白质序列与已知的53个耐铬真菌和细菌菌株的蛋白质序列进行了比较分析。使用ProtParam工具分析已知和分离的抗铬蛋白的物理和化学参数。利用EMBOSS-NEEDLE和FATCAT分别对已知和分离的耐铬蛋白序列和结构比对进行了比较研究。结果:在本分析中,报道了与A. proliferans (NCIM-1473)相似性最高的10株菌株,其中铬调控蛋白Cupriavidus metallidurans CH34与分离的抗铬蛋白在ProtParam、sequence、FATCAT分析中均具有最大的相似性。据报道,该菌株Cupriavidus metallidurans CH34对8种重金属(其中一种是铬酸盐)具有抗性,并首次在比利时的一个沉淀池中被重金属污染的污泥中发现。结论:从本实验室分离到的菌株(菌株编号:1)NCIM-1473)具有潜在的抗铬性,是一种独特的菌株。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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