Gene enrichment analysis and protein–protein interaction network topology delineates S-Phase kinase-associated protein 1 and catenin beta-1 as potential signature genes linked to glioblastoma prognosis

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-01-01 DOI:10.4103/bbrj.bbrj_344_22

K. Ashwini, Pavan Gollapalli, S. Shetty, A. Raghotham, P. Shetty, Jayaprakash Shetty, N. Kumari

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引用次数: 0

Abstract

Background: Glioblastoma (GBM) is the most malignant and accounts for 60% of brain tumors in adults. Current therapy for GBM involves surgical removal of the tumor followed by radiotherapy with concomitant adjuvant therapy temozolomide. Despite improvements in therapy, patient survival remains low. The exact etiology of a brain tumor is uncertain, and numerous unknown genes are involved in the progression of GBM. The aim of the present study was to evaluate various genes involved in GBM through bioinformatic approach. Methods: In the present study, gene expression profile of GSE68424 was retrieved from the GEO database to explore the genes in GBM. Results: Analysis of expression profile data revealed that 33 genes were upregulated and 1189 genes were downregulated based on the log2 fold change cut-off criteria. The genes S-Phase kinase-associated protein 1 (SKP1) and Catenin beta-1 (CTNNB1) have been linked to GBM prognosis. Conclusion: SKP1 and CTNNB1 were identified as a candidate gene for GBM study as a result of these findings. Catenin beta-1 was the protein with the highest closeness centrality value and is the key component of canonical Wnt signaling downstream pathway. More study is needed to establish the molecular function of SKP1 and CTNNB1 in GBM development, as well as the biomarker's specificity and sensitivity.

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基因富集分析和蛋白相互作用网络拓扑描述了s期激酶相关蛋白1和连环蛋白β -1是与胶质母细胞瘤预后相关的潜在标志基因

背景:胶质母细胞瘤(GBM)是恶性程度最高的肿瘤，占成人脑肿瘤的60%。目前治疗GBM的方法包括手术切除肿瘤，放疗配合替莫唑胺辅助治疗。尽管治疗有所改善，但患者存活率仍然很低。脑肿瘤的确切病因尚不确定，许多未知基因参与了GBM的进展。本研究的目的是通过生物信息学方法评估与GBM有关的各种基因。方法:本研究从GEO数据库中检索GSE68424基因表达谱，探索GBM基因。结果:表达谱数据分析显示，基于log2倍变化截止标准，33个基因表达上调，1189个基因表达下调。基因s期激酶相关蛋白1 (SKP1)和Catenin β -1 (CTNNB1)与GBM预后有关。结论:SKP1和CTNNB1是GBM研究的候选基因。Catenin β -1是接近中心性值最高的蛋白，是典型Wnt信号下游通路的关键组分。需要更多的研究来确定SKP1和CTNNB1在GBM发生中的分子功能，以及生物标志物的特异性和敏感性。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.