Unraveling the pathways influencing the berry color and firmness of grapevine cv. Flame Seedless treated with bioregulators using biochemical and RNA-Seq analysis under semi-arid subtropics

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

Food Chemistry Molecular Sciences Pub Date : 2022-12-30 DOI:10.1016/j.fochms.2022.100116

Vishal B. Mhetre , V.B. Patel , S.K. Singh , Gyan P. Mishra , M.K. Verma , Chavlesh Kumar , Anil Dahuja , Sanjeev Kumar , Rakesh Singh , M. Wasim Siddiqui

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引用次数: 0

Abstract

Plant bioregulators (PBRs) regulate developmental and physiological processes in plants. In this study, biochemical and transcriptomic analyses were conducted to evaluate the influence of PBRs [abscisic acid (ABA), benzothiadiazole (BTH), ethephon, and prohexadione-calcium (Pro-Ca)] on the grapevine cv. Flame Seedless under semi-arid subtropics. This study aims to see the effect of exogenous application of PBRs on overall berry quality, including uniformity of berry color. Uniform colored berries, the maximum total soluble solids (TSS) and total antioxidant activity (TAoA), and the highest total phenolics (TPC) and flavonoids (TFC) contents were obtained with the treatments, namely, 400 mg L^-1 ethephon and 400 mg L^-1 ABA. Further, RNA-Seq analysis has also identified some key DEGs like UFGT (VIT_16s0039g02230), GST (VIT_04s0079g00690), and chalcone synthase (CHS) (VIT_05s0136g00260) which were part of the anthocyanin biosynthesis pathway controlling grape berries color. Thus, ethephon (400 mg L^-1) and ABA (400 mg L^-1) were found promising for attaining greater uniformity in berry color development because of increased total anthocyanins content. In addition, they were also found associated with enhanced TAoA, TPC, and TFC. Hence, ethephon and ABA can be recommended for improving the berry quality.

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揭示影响葡萄果实颜色和硬度的途径。半干旱亚热带地区用生物调节剂处理火焰无籽，采用生化和RNA-Seq分析

植物生物调节剂(PBRs)调节植物的发育和生理过程。本研究通过生化和转录组学分析来评估pbr[脱落酸(ABA)、苯并噻唑(BTH)、乙烯利和prohexadione-calcium (Pro-Ca)]对葡萄cv的影响。半干旱亚热带地区无籽。本研究旨在了解外源施用pbr对浆果整体品质的影响，包括浆果颜色均匀性。400 mg L-1乙烯利和400 mg L-1 ABA处理的果实颜色均匀，总可溶性固形物(TSS)和总抗氧化活性(TAoA)最高，总酚(TPC)和总黄酮(TFC)含量最高。此外，RNA-Seq分析还鉴定出一些关键的基因，如UFGT (VIT_16s0039g02230)、GST (VIT_04s0079g00690)和查尔酮合成酶(CHS) (VIT_05s0136g00260)，它们是控制葡萄果实颜色的花青素生物合成途径的一部分。因此，乙烯利(400 mg L-1)和ABA (400 mg L-1)被发现有希望获得更均匀的浆果颜色发育，因为增加了总花青素含量。此外，还发现它们与TAoA、TPC和TFC增强有关。因此，乙烯利和ABA可作为提高浆果品质的有效添加剂。

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来源期刊

Food Chemistry Molecular Sciences Agricultural and Biological Sciences-Food Science

CiteScore

6.00

自引率

0.00%

发文量

审稿时长

82 days

期刊介绍： Food Chemistry: Molecular Sciences is one of three companion journals to the highly respected Food Chemistry. Food Chemistry: Molecular Sciences is an open access journal publishing research advancing the theory and practice of molecular sciences of foods. The types of articles considered are original research articles, analytical methods, comprehensive reviews and commentaries. Topics include: Molecular sciences relating to major and minor components of food (nutrients and bioactives) and their physiological, sensory, flavour, and microbiological aspects; data must be sufficient to demonstrate relevance to foods and as consumed by humans Changes in molecular composition or structure in foods occurring or induced during growth, distribution and processing (industrial or domestic) or as a result of human metabolism Quality, safety, authenticity and traceability of foods and packaging materials Valorisation of food waste arising from processing and exploitation of by-products Molecular sciences of additives, contaminants including agro-chemicals, together with their metabolism, food fate and benefit: risk to human health Novel analytical and computational (bioinformatics) methods related to foods as consumed, nutrients and bioactives, sensory, metabolic fate, and origins of foods. Articles must be concerned with new or novel methods or novel uses and must be applied to real-world samples to demonstrate robustness. Those dealing with significant improvements to existing methods or foods and commodities from different regions, and re-use of existing data will be considered, provided authors can establish sufficient originality.