Effect of lncRNA H19 on nerve degeneration and regeneration after sciatic nerve injury in rats

IF 2.7 4区医学 Q2 DEVELOPMENTAL BIOLOGY

Developmental Neurobiology Pub Date : 2021-11-24 DOI:10.1002/dneu.22861

Yuting Li, Min Cai, Yumei Feng, Bryant Yung, Yi Wang, Nannan Gao, Xi Xu, Huanhuan Zhang, Huiwei Huang, Dengbing Yao

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引用次数: 4

Abstract

Hundreds of millions of people worldwide suffer from peripheral nerve damage resulting from car accidents, falls, industrial accidents, residential accidents, and wars. The purpose of our study was to further investigate the effects of Wallerian degeneration (WD) after rat sciatic nerve injury and to screen for critical long noncoding RNAs (lncRNAs) in WD. We found H19 to be essential for nerve degeneration and regeneration and to be highly expressed in the sciatic nerves of rats with WD. lncRNA H19 potentially impaired the recovery of sciatic nerve function in rats. H19 was mainly localized in the cytoplasm of Schwann cells (SCs) and promoted their migration. H19 promoted the apoptosis of dorsal root ganglion (DRG) neurons and slowed the growth of DRG axons. The lncRNA H19 may play a role in WD through the Wnt/β-catenin signaling pathway and is coexpressed with a variety of crucial mRNAs during WD. These data provide further insight into the molecular mechanisms of WD.

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lncRNA H19对大鼠坐骨神经损伤后神经变性和再生的影响

全世界有数亿人因车祸、跌倒、工业事故、居住事故和战争而遭受周围神经损伤。我们的研究目的是进一步探讨大鼠坐骨神经损伤后沃勒氏变性(WD)的影响，并筛选关键的长链非编码rna (lncRNAs)。我们发现H19对WD大鼠的神经变性和再生至关重要，并在坐骨神经中高表达。lncRNA H19可能会损害大鼠坐骨神经功能的恢复。H19主要定位于雪旺细胞(SCs)的细胞质中，并促进其迁移。H19促进背根神经节(DRG)神经元凋亡，减缓DRG轴突生长。lncRNA H19可能通过Wnt/β-catenin信号通路在WD中发挥作用，并在WD中与多种关键mrna共表达。这些数据为WD的分子机制提供了进一步的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Developmental Neurobiology 生物-发育生物学

CiteScore

6.50

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Developmental Neurobiology (previously the Journal of Neurobiology ) publishes original research articles on development, regeneration, repair and plasticity of the nervous system and on the ontogeny of behavior. High quality contributions in these areas are solicited, with an emphasis on experimental as opposed to purely descriptive work. The Journal also will consider manuscripts reporting novel approaches and techniques for the study of the development of the nervous system as well as occasional special issues on topics of significant current interest. We welcome suggestions on possible topics from our readers.