Maturation of NMDA receptor-mediated spontaneous postsynaptic currents in the rat locus coeruleus neurons.

IF 2.2 4区医学 Q3 PHYSIOLOGY

Physiology international Pub Date : 2020-04-23 Print Date: 2020-03-01 DOI:10.1556/2060.2020.00010

M Kourosh-Arami, S Hajizadeh

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引用次数: 1

Abstract

Introduction: During mammalian brain development, neural activity leads to maturation of glutamatergic innervations to locus coeruleus. In this study, fast excitatory postsynaptic currents mediated by N-methyl-d-aspartate (NMDA) receptors were evaluated to investigate the maturation of excitatory postsynaptic currents in locus coeruleus (LC) neurons.

Methods: NMDA receptor-mediated synaptic currents in LC neurons were evaluated using whole-cell voltage-clamp recording during the primary postnatal weeks. This technique was used to calculate the optimum holding potential for NMDA receptor-mediated currents and the best frequency for detecting spontaneous excitatory postsynaptic currents (sEPSC).

Results: The optimum holding potential for detecting NMDA receptor-mediated currents was + 40 to + 50 mV in LC neurons. The frequency, amplitude, rise time, and decay time constant of synaptic responses depended on the age of the animal and increased during postnatal maturation.

Conclusion: These findings suggest that most nascent glutamatergic synapses express functional NMDA receptors in the postnatal coerulear neurons, and that the activities of the neurons in this region demonstrate an age-dependent variation.

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大鼠蓝斑神经元NMDA受体介导的自发突触后电流的成熟。

在哺乳动物的大脑发育过程中，神经活动导致了蓝斑的谷氨酸神经支配的成熟。本研究利用n -甲基-d-天冬氨酸(NMDA)受体介导的快速兴奋性突触后电流，研究蓝斑(LC)神经元兴奋性突触后电流的成熟情况。方法:采用全细胞电压钳记录法观察NMDA受体介导的LC神经元突触电流。该技术用于计算NMDA受体介导电流的最佳保持电位和检测自发兴奋性突触后电流(sEPSC)的最佳频率。结果:LC神经元检测NMDA受体介导电流的最佳保持电位为+ 40 ~ + 50 mV。突触反应的频率、幅度、上升时间和衰减时间常数与动物的年龄有关，在出生后成熟时增加。结论:这些发现表明，大多数新生的谷氨酸能突触在出生后的蓝核神经元中表达功能性的NMDA受体，并且该区域的神经元活动表现出年龄依赖性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Physiology international Medicine-Physiology (medical)

CiteScore

3.40

自引率

0.00%

发文量

期刊介绍： The journal provides a forum for important new research papers written by eminent scientists on experimental medical sciences. Papers reporting on both original work and review articles in the fields of basic and clinical physiology, pathophysiology (from the subcellular organization level up to the oranizmic one), as well as related disciplines, including history of physiological sciences, are accepted.