Use of ionic liquid for X-ray micro-CT specimen preparation of imbibed seeds

IF 1.8 4区工程技术

Microscopy Pub Date : 2019-02-01 DOI:10.1093/jmicro/dfy130

Daisuke Yamauchi;Aki Fukuda;Tomonori Nakai;Ichirou Karahara;Miyuki Takeuchi;Daisuke Tamaoki;Tetsuya Tsuda;Katsuhiko Tsunashima;Susumu Kuwabata;Masato Hoshino;Kentaro Uesugi;Akihisa Takeuchi;Yoshio Suzuki;Yoshinobu Mineyuki

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引用次数: 2

Abstract

We developed a novel specimen preparation method for X-ray micro-CT observation of wet biological samples using ionic liquids. Treatment with high concentrations of ionic liquids after osmium tetroxide fixation helped not only to prevent shrinkage but also to maintain the cellular architecture of imbibed Arabidopsis seeds. X-ray micro-CT is one of the most useful techniques to examine 3D cellular architecture inside dry seeds. However, the examination of imbibed seeds is difficult because immersion in water causes a decline in the image quality. Here, we examined the use of ionic liquids for specimen preparation of chemically fixed imbibed seeds of Arabidopsis. We found that treatment with high concentrations of ionic liquids after osmium tetroxide fixation helped not only to prevent the structural damage caused by seed shrinkage, but also to preserve the image quality. Under these conditions, the cellular architecture of seeds was also well maintained.

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离子液体在吸收种子X射线显微CT标本制备中的应用

我们开发了一种新的样品制备方法，用于使用离子液体对湿生物样品进行X射线显微CT观察。四氧化锇固定后用高浓度离子液体处理不仅有助于防止收缩，而且有助于维持吸收的拟南芥种子的细胞结构。X射线显微CT是检测干种子内部三维细胞结构最有用的技术之一。然而，由于浸泡在水中会导致图像质量下降，因此对吸收的种子进行检查是困难的。在这里，我们研究了离子液体用于化学固定的拟南芥吸收种子的样品制备。我们发现，四氧化锇固定后用高浓度离子液体处理不仅有助于防止种子收缩引起的结构损伤，而且有助于保持图像质量。在这些条件下，种子的细胞结构也得到了很好的维持。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microscopy 工程技术-显微镜技术

自引率

11.10%

发文量

审稿时长

>12 weeks

期刊介绍： Microscopy, previously Journal of Electron Microscopy, promotes research combined with any type of microscopy techniques, applied in life and material sciences. Microscopy is the official journal of the Japanese Society of Microscopy.