Low Expression of LEFTY1 in Placental Villi Is Associated with Early Unexplained Miscarriage.

IF 0.2 4区医学 Q4 Medicine

生殖医学杂志 Pub Date : 2017-05-01

Huiqin Xue, Lan Ma, Jinjie Xue, Xiayu Sun, Ming Li, Hongyong Lu, Yan Zhou, Yuezhen Guo, Yuping Zhang, Jigeng Bai

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引用次数: 0

Abstract

OBJECTIVE: To investigate the function and underlying mechanism of transforming growth factor–beta (TGF-β)/bone morphogenetic protein (BMP) signaling pathway in early unexplained miscarriage. STUDY DESIGN: Expression profiles of genes involved in TGF-β/BMP signaling pathway were compared between placental villous tissue samples from 2 women with missed abortion and those from 2 women with induced abortion by microarray assay. The protein expression level of the most downregulated gene—LEFTY1—was further measured using western blotting in another 8 women with missed abortion and 7 women with induced abortion. RESULTS: A total of 24 genes showed differential expression level between the 2 groups. Their functions were further investigated, of which 6 of 13 upregulated genes were TGF-β responsive genes. The most reduced gene is LEFTY1, an antagonist of TGF-β ligand. The protein expression level of LEFTY1 was confirmed to show the same trend as microarray using western blotting. CONCLUSION: A reduced expression of LEFTY1 in women with missed abortion was identified as com-pared with women with induced abortion, which may result in a dysregulation of TGF-β signaling and may be the underlying mechanism of missed abortion.

本刊更多论文

胎盘绒毛LEFTY1低表达与早期不明原因流产有关

目的:探讨转化生长因子-β (TGF-β)/骨形态发生蛋白(BMP)信号通路在早期不明原因流产中的作用及其机制。研究设计:采用微阵列法比较2例流产患者和2例人工流产患者胎盘绒毛组织中TGF-β/BMP信号通路相关基因的表达谱。在另外8例流产妇女和7例人工流产妇女中，进一步采用western blotting检测下调幅度最大的基因lefty1的蛋白表达水平。结果:两组间共有24个基因表达水平存在差异。我们进一步研究了它们的功能，13个上调基因中有6个是TGF-β应答基因。减少最多的基因是LEFTY1，它是TGF-β配体的拮抗剂。western blotting证实LEFTY1蛋白表达水平与芯片表达趋势一致。结论:与人工流产患者相比，LEFTY1在漏流产患者中表达降低，这可能导致TGF-β信号的失调，可能是漏流产的潜在机制。

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来源期刊

生殖医学杂志医学-妇产科学

自引率

0.00%

发文量

6427

审稿时长

6-12 weeks

期刊介绍： The Journal of Reproductive Medicine® has been the essential tool of Obstetricians and Gynecologists since 1968. As a highly regarded professional journal and the official periodical of six medical associations, JRM® brings timely and relevant information on the latest procedures and advances in the field of reproductive medicine. Published bimonthly, JRM® contains peer-reviewed articles and case reports submitted by top specialists. Common topics include research, clinical practice, and case reports related to general obstetrics and gynecology, infertility, female cancers, gynecologic surgery, contraception, and medical education.