Measurement of Absorption and Scattering With an Integrating Sphere Detector: Application to Microalgae.

IF 1.5 4区工程技术

Journal of Research of the National Institute of Standards and Technology Pub Date : 2009-04-01 Print Date: 2009-03-01 DOI:10.6028/jres.114.006

A K Gaigalas, Hua-Jun He, Lili Wang

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引用次数: 28

Abstract

A spectrometer with an integrating sphere (IS) detector was used to measure the absorbance due to scattering and absorption. Analysis of the measurement process showed that two measurements of the absorbance, one with the cuvette placed in the normal spectrometer position, and the second with the cuvette placed next to the entrance aperture of the IS detector, provide enough information to separate the contributions from scattering and molecular absorption. Measurements were carried out with mixtures of microsphere and chromophore solutions. Two cases were examined: microspheres suspended in an aqueous fluorescein solution, and microspheres suspended in an aqueous holmium oxide solution. In both cases, the proposed measurement model gave results which were in good agreement with the expected response. Measurements on microalgae suspensions yielded a molecular absorption contribution and a scattering contribution. The scattering contribution had significant spectral structure which was inversely related to the molecular absorption contribution. The absorption and scattering contributions may provide independent information on the status of chlorophyll molecules and the structure of chloroplasts in microalgae.

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积分球探测器的吸收散射测量:在微藻中的应用。

采用积分球(IS)探测器的光谱仪测量了由于散射和吸收引起的吸光度。测量过程分析表明，两次吸光度测量，一次将比色皿放置在光谱仪正常位置，第二次将比色皿放置在IS探测器的入口孔旁边，提供了足够的信息来分离散射和分子吸收的贡献。用微球和发色团溶液的混合物进行测量。研究了两种情况:微球悬浮在荧光素水溶液中，微球悬浮在氧化钬水溶液中。在这两种情况下，所提出的测量模型给出的结果与预期的响应很好地一致。对微藻悬浮液的测量得出了分子吸收贡献和散射贡献。散射贡献具有显著的光谱结构，与分子吸收贡献呈负相关。吸收和散射贡献可以为微藻叶绿素分子的状态和叶绿体结构提供独立的信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Research of the National Institute of Standards and Technology Engineering-General Engineering

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33.30%

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期刊介绍： The Journal of Research of the National Institute of Standards and Technology is the flagship publication of the National Institute of Standards and Technology. It has been published under various titles and forms since 1904, with its roots as Scientific Papers issued as the Bulletin of the Bureau of Standards. In 1928, the Scientific Papers were combined with Technologic Papers, which reported results of investigations of material and methods of testing. This new publication was titled the Bureau of Standards Journal of Research. The Journal of Research of NIST reports NIST research and development in metrology and related fields of physical science, engineering, applied mathematics, statistics, biotechnology, information technology.