Development, validation, and uncertainty measurement of HPLC-DAD method for determination of some free amino acids in infant formula and medical food products for inborn errors of metabolism

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2022-10-01 DOI:10.1016/j.foodchem.2022.133204

Hadis Vakili , Zahra Talebpour , Farideh Haghighi

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引用次数: 5

Abstract

This research aims at determining some free amino acids in amino acid-based infant formulas and amino acid-modified medical foods for inborn errors of metabolism to prove their quality. A method based on high-performance liquid chromatography and diode array detection was developed and validated. Then, overall uncertainty was estimated by the bottom-up approach. Applying the weighted least squares regression method suggested good linearity with coefficient of determinations ≥ 0.9960. The limits of detection were calculated between 0.01 and 0.28 μg/mL. The most repetitive recovery values were obtained in the range of 91–108%, with RSDs ≤ 15%. The expanded uncertainties were below 20% for most amino acids. The contributions of linear regression and repeatability are two main factors in estimating overall uncertainty. The results offer this method as a simple and easy procedure for analyzing free amino acids in seven powdered medical foods designed for phenylketonuria, maple syrup urine disease, methylmalonic, and propionic acidemia.

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HPLC-DAD法测定婴儿配方奶粉和医疗食品中某些游离氨基酸的不确定度及验证

本研究旨在测定以氨基酸为基础的婴儿配方奶粉和用于先天性代谢错误的氨基酸修饰医疗食品中的部分游离氨基酸，以证明其质量。建立了一种基于高效液相色谱和二极管阵列检测的方法并进行了验证。然后，采用自底向上的方法估计总体不确定性。采用加权最小二乘回归方法，线性良好，决定系数≥0.9960。检出限为0.01 ~ 0.28 μg/mL。在91 ~ 108%范围内重复性最高，rsd≤15%。大多数氨基酸的扩展不确定度在20%以下。线性回归和可重复性的贡献是估计总体不确定性的两个主要因素。结果表明，该方法可用于苯丙酮尿症、枫糖尿病、甲基丙二酸和丙酸血症等7种粉状医用食品中游离氨基酸的分析。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.