Suspended, micron-scale corner cube retroreflectors as ultra-bright optical labels.

IF 1.5 4区 工程技术 Q3 ENGINEERING, ELECTRICAL & ELECTRONIC
Tim Sherlock, Azeem Nasrullah, Julia Litvinov, Eliedonna Cacao, Jennifer Knoop, Steven Kemper, Katerina Kourentzi, Archana Kar, Paul Ruchhoeft, Richard Willson
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引用次数: 12

Abstract

Corner cube retroreflectors are objects with three mutually perpendicular reflective surfaces that return light directly to its source and are therefore extremely bright and easy to detect. In this work, we have fabricated suspended corner cube retroreflectors, 5 microns in size, consisting of a transparent epoxy core and three surfaces coated with gold as ultra-bright labels for use in a rapid, low-labor diagnostic platform. The authors have demonstrated that individual cubes are easily imaged using low-cost, low numerical aperture objectives in suspension and that they remain suspended over long periods of time. Moreover, we have demonstrated that the gold outer surfaces can be decorated with proteins, and that individual cubes can be bound to magnetic sample preparation particles bearing antibodies which recognize these proteins. The bound cubes can be imaged and tracked as they move through solution in response to an external magnetic field, and we have, as such, demonstrated the principle of the new biosensing approach.

Abstract Image

悬挂,微米尺度的角立方反光镜作为超亮光学标签。
角立方反反射器是具有三个相互垂直的反射表面的物体,它们将光线直接返回到光源,因此非常明亮,易于检测。在这项工作中,我们制作了悬挂的角立方反反射器,大小为5微米,由透明环氧树脂芯和三个表面涂有金色作为超亮标签,用于快速,低人工诊断平台。作者已经证明,使用低成本、低数值孔径的悬浮物镜,单个立方体很容易成像,并且它们在很长一段时间内保持悬浮。此外,我们已经证明,金的外表面可以用蛋白质修饰,并且单个立方体可以与带有识别这些蛋白质的抗体的磁性样品制备颗粒结合。当结合的立方体响应于外部磁场在溶液中移动时,可以对其进行成像和跟踪,因此,我们已经证明了新的生物传感方法的原理。
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来源期刊
CiteScore
2.70
自引率
0.00%
发文量
146
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