Effects of Addition of Mannitol Crystals on the Porosity and Dissolution Rates of a Calcium Phosphate Cement.

IF 1.5 4区 工程技术
Debra Vazquez, Shozo Takagi, Stan Frukhtbeyn, Laurence C Chow
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引用次数: 8

Abstract

The bone defect repair functions of calcium phosphate cement (CPC) are related to its osteoconductivity and its gradual replacement by new bone. Adding mannitol to CPC may enhance its bone repair potential by increasing CPCs macroporosity and dissolution rate. The objective of the study was to assess microporosity and macroporosity and dissolution rates for CPC mixed with mannitol. Three groups of CPC discs were prepared by combining an equimolar mixture of tetracalcium phosphate and anhydrous dicalcium phosphate with (0 %, 10 %, or 50 %) mass fraction (hereafter expressed as mass %) of mannitol. Macroporosity and microporosity of the samples were calculated from volume and mass measurements of the discs. Discs were then placed in a pH 3.0 demineralizing solution simulating acidified physiological solution, and dissolution rates were measured by a previously described constant-composition titration method. Pure CPC exhibited no macropores and microporosity (mean ± s.d.; n = 5) of (46.8 ± 0.8) % volume fraction (hereafter expressed as vol %). Adding 10 mass % mannitol resulted in 15.6 ± 3.9 vol % macroporosity and 39.4 ± 1.8 vol % microporosity, and adding 50 mass % mannitol produced 54.7 ± 0.8 vol % macroporosity and 21.1 ± 0.4 vol % microporosity. The dissolution rates (mean ± s.d.; n = 5) of CPC with (0, 10, and 50) mass % mannitol incorporation were (30.6 ± 3.4, 44.8 ± 10.2, and 54.7 ± 3.6, respectively) μg · cm(-2) · min(-1), or (0.018 ± 0.002, 0.032 ± 0.007, and 0.072 ± 0.005, respectively) μL · cm(-2) · min(-1). Adding either 10 mass % or 50 mass % mannitol into CPC significantly (p < 0.05) increased CPC dissolution rates. Adding mannitol readily increased macroporosity and dissolution rate of CPC, which may enhance the capacity of CPC to be osteoconductive.

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Abstract Image

Abstract Image

甘露醇晶体的加入对磷酸钙水泥孔隙率和溶解速率的影响。
磷酸钙骨水泥(CPC)的骨缺损修复功能与其骨传导能力及其逐渐被新骨替代有关。甘露醇可通过增加CPCs的宏观孔隙度和溶出速率来增强CPCs的骨修复能力。该研究的目的是评估混合甘露醇的CPC的微孔隙率和宏观孔隙率以及溶出率。将磷酸四钙和无水磷酸二钙的等摩尔混合物与甘露醇(0%、10%、50%)质量分数(以下以质量%表示)混合制备三组CPC圆盘。通过测量圆盘的体积和质量,计算了试样的宏观孔隙度和微观孔隙度。然后将圆盘放入pH 3.0的模拟酸化生理溶液中,通过先前描述的恒成分滴定法测量溶解速率。纯CPC无宏观孔隙和微观孔隙(平均±s.d;N = 5)为(46.8±0.8)%体积分数(以下以vol %表示)。添加10质量%甘露醇的孔隙率为15.6±3.9 vol %,微孔隙率为39.4±1.8 vol %;添加50质量%甘露醇的孔隙率为54.7±0.8 vol %,微孔隙率为21.1±0.4 vol %。溶出率(平均±s.d;n = 5),甘露醇掺入(0、10、50)质量%的CPC分别为(30.6±3.4、44.8±10.2、54.7±3.6)μL·cm(-2)·min(-1)或(0.018±0.002、0.032±0.007、0.072±0.005)μL·cm(-2)·min(-1)。添加10%质量%和50%质量%的甘露醇可显著提高CPC的溶出率(p < 0.05)。甘露醇的加入增加了CPC的宏观孔隙率和溶解速率,从而增强了CPC的骨传导能力。
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来源期刊
自引率
33.30%
发文量
10
期刊介绍: The Journal of Research of the National Institute of Standards and Technology is the flagship publication of the National Institute of Standards and Technology. It has been published under various titles and forms since 1904, with its roots as Scientific Papers issued as the Bulletin of the Bureau of Standards. In 1928, the Scientific Papers were combined with Technologic Papers, which reported results of investigations of material and methods of testing. This new publication was titled the Bureau of Standards Journal of Research. The Journal of Research of NIST reports NIST research and development in metrology and related fields of physical science, engineering, applied mathematics, statistics, biotechnology, information technology.
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