In Vivo Characteristics of Premixed Calcium Phosphate Cements When Implanted in Subcutaneous Tissues and Periodontal Bone Defects.

IF 1.5 4区 工程技术
Akiyoshi Sugawara, Kenji Fujikawa, Satoshi Hirayama, Shozo Takagi, Laurence C Chow
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引用次数: 0

Abstract

Previous studies showed that water-free, premixed calcium phosphate cements (Pre-CPCs) exhibited longer hardening times and lower strengths than conventional CPCs, but were stable in the package. The materials hardened only after being delivered to a wet environment and formed hydroxyapatite as the only product. Pre-CPCs also demonstrated good washout resistance and excellent biocompatibility when implanted in subcutaneous tissues in rats. The present study evaluated characteristics of Pre-CPCs when implanted in subcutaneous tissues (Study I) and used for repairing surgically created two-wall periodontal defects (Study II). Pre-CPC pastes were prepared by combining CPC powders that consisted of CPC-1: Ca(4)(PO(4))(2)O and CaHPO(4), CPC-2: α-Ca(3)(PO(4))(2) and CaCO(3) or CPC-3: DCPA and Ca(OH)(2) with a glycerol at powder-to-liquid mass ratios of 3.5, 2.5, and 2.5, respectively. In each cement mixture, the Ca to P molar ratio was 1.67. The glycerol contained Na(2)HPO(4) (30 mass %) and hydroxypropyl methylcellulose (0.55 %) to accelerate cement hardening and improve washout resistance, respectively. In Study I, the test materials were implanted subcutaneously in rats. Four weeks after the operation, the animals were sacrificed and histopathological observations were performed. The results showed that all of the implanted materials exhibited very slight or negligible inflammatory reactions in tissues contacted with the implants. In Study II, the mandibular premolar teeth of mature beagle dogs were extracted. One month later, two-wall periodontal bone defects were surgically created adjacent to the teeth of the mandibular bone. The defects were filled with the Pre-CPC pastes and the flaps replaced in the preoperative position. The dogs were sacrificed at 1, 3 and 6 months after surgery and sections of filled defects resected. Results showed that one month after surgery, the implanted Pre-CPC-1 paste was partially replaced by bone and was converted to bone at 6 months. The pockets filled with Pre-CPC-2 were completely covered by newly formed bone in 1 month. The Pre-CPC-2 was partially replaced by trabecular bone in 1 month and was completely replaced by bone in 6 months. Examination of 1 month and 3 month samples indicated that Pre-CPC-2 resorbed and was replaced by bone more rapidly than Pre-CPC 1. Both Pre-CPC pastes were highly osteoconductive. When implanted in periodontal defects, Pre-CPC-2 was replaced by bone more rapidly than Pre-CPC-1.

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植入皮下组织和牙周骨缺损处的预混合磷酸钙粘合剂的体内特性
以前的研究表明,与传统的磷酸钙水门汀相比,无水预混磷酸钙水门汀(Pre-CPCs)的硬化时间更长,强度更低,但在包装中却很稳定。这些材料只有在进入潮湿环境后才会硬化,并形成羟基磷灰石作为唯一的产物。将 Pre-CPCs 植入大鼠皮下组织时,还表现出良好的耐冲刷性和出色的生物相容性。本研究评估了将 Pre-CPCs 植入皮下组织(研究 I)和用于修复手术造成的双壁牙周缺损(研究 II)时的特性。将由 CPC-1:Ca(4)(PO(4))(2)O 和 CaHPO(4)、CPC-2:α-Ca(3)(PO(4))(2) 和 CaCO(3) 或 CPC-3:DCPA 和 Ca(OH)(2)组成的 CPC 粉末与甘油混合,粉液质量比分别为 3.5、2.5 和 2.5,制备 Pre-CPC 浆料。在每种水泥混合物中,Ca 与 P 的摩尔比均为 1.67。甘油中含有 Na(2)HPO(4) (30 质量 %) 和羟丙基甲基纤维素 (0.55 %),分别用于加速水泥硬化和提高抗冲刷性。在研究 I 中,试验材料被植入大鼠皮下。手术四周后,动物被处死并进行组织病理学观察。结果显示,所有植入材料在与植入物接触的组织中都表现出非常轻微或可以忽略不计的炎症反应。在研究 II 中,成熟的小猎犬下颌前磨牙被拔除。一个月后,通过手术在下颌骨牙齿附近形成两壁牙周骨缺损。用 Pre-CPC 粘贴剂填充缺损,并在术前位置替换牙瓣。狗在术后 1、3 和 6 个月被处死,并切除已填充缺损的部分。结果显示,术后 1 个月,植入的 Pre-CPC-1 粘贴部分被骨取代,6 个月后转变为骨。用 Pre-CPC-2 填充的凹陷在 1 个月后完全被新形成的骨覆盖。1 个月后,Pre-CPC-2 部分被骨小梁取代,6 个月后完全被骨取代。对 1 个月和 3 个月样本的检查表明,Pre-CPC-2 比 Pre-CPC 1 更快吸收并被骨取代。当植入牙周缺损处时,Pre-CPC-2 比 Pre-CPC-1 更快地被骨取代。
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来源期刊
自引率
33.30%
发文量
10
期刊介绍: The Journal of Research of the National Institute of Standards and Technology is the flagship publication of the National Institute of Standards and Technology. It has been published under various titles and forms since 1904, with its roots as Scientific Papers issued as the Bulletin of the Bureau of Standards. In 1928, the Scientific Papers were combined with Technologic Papers, which reported results of investigations of material and methods of testing. This new publication was titled the Bureau of Standards Journal of Research. The Journal of Research of NIST reports NIST research and development in metrology and related fields of physical science, engineering, applied mathematics, statistics, biotechnology, information technology.
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