Effect and mechanism of functional compound fruit drink on gut microbiota in constipation mice

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2023-02-01 DOI:10.1016/j.foodchem.2022.134210

Yali Shi , Fei Chen , Ziqi Wang , Jun Cao , Chuan Li

{"title":"Effect and mechanism of functional compound fruit drink on gut microbiota in constipation mice","authors":"Yali Shi , Fei Chen , Ziqi Wang , Jun Cao , Chuan Li","doi":"10.1016/j.foodchem.2022.134210","DOIUrl":null,"url":null,"abstract":"<div><p>Compound fruit drink (CFD) is a functional drink prepared with fruit, Chinese herbs and prebiotic fructooligosaccharide as the main ingredients. Loperamide hydrochloride was used to establish a mouse model of constipation. And the effect of CFD on the improvement of constipation and the impact on gut microbiota were studied. The results showed that CFD significantly enhanced intestinal motility in constipated mice (<em>P</em> < 0.05). It significantly improved serum levels of gastrointestinal regulatory-related peptides, elevated the short-chain fatty acids (SCFAs) content and alleviated colonic injury. Meanwhile, CFD also up-regulated the mRNA expression levels of AQP3, AQP9, SCF and c-Kit and the related protein expression levels. Fecal microbial results showed that the CFD medium-dose group significantly increased species richness. Furthermore, CFD increased the abundance of potentially beneficial bacteria and reduced the number of potentially pathogenic bacteria. This study indicated that CFD was a promising functional drink for effectively relieving constipation.</p></div>","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"401 ","pages":"Article 134210"},"PeriodicalIF":9.8000,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"8","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0308814622021720","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}

引用次数: 8

Abstract

Compound fruit drink (CFD) is a functional drink prepared with fruit, Chinese herbs and prebiotic fructooligosaccharide as the main ingredients. Loperamide hydrochloride was used to establish a mouse model of constipation. And the effect of CFD on the improvement of constipation and the impact on gut microbiota were studied. The results showed that CFD significantly enhanced intestinal motility in constipated mice (P < 0.05). It significantly improved serum levels of gastrointestinal regulatory-related peptides, elevated the short-chain fatty acids (SCFAs) content and alleviated colonic injury. Meanwhile, CFD also up-regulated the mRNA expression levels of AQP3, AQP9, SCF and c-Kit and the related protein expression levels. Fecal microbial results showed that the CFD medium-dose group significantly increased species richness. Furthermore, CFD increased the abundance of potentially beneficial bacteria and reduced the number of potentially pathogenic bacteria. This study indicated that CFD was a promising functional drink for effectively relieving constipation.

查看原文本刊更多论文

功能性复合果汁饮料对便秘小鼠肠道菌群的影响及机制

复合水果饮料(CFD)是以水果、中草药和益生元低聚果糖为主要成分配制而成的功能性饮料。采用盐酸洛哌丁胺建立小鼠便秘模型。研究了CFD对便秘的改善作用及对肠道菌群的影响。结果表明，CFD可显著增强便秘小鼠的肠道蠕动(P <0.05)。显著改善血清胃肠调节相关肽水平，提高短链脂肪酸(SCFAs)含量，减轻结肠损伤。同时，CFD还上调了AQP3、AQP9、SCF和c-Kit mRNA表达水平及相关蛋白表达水平。粪便微生物结果显示，CFD中剂量组显著增加了物种丰富度。此外，CFD增加了潜在有益细菌的丰度，减少了潜在致病菌的数量。本研究提示CFD是一种有前景的功能饮料，可以有效缓解便秘。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.