Metabolic and transcriptomic analyses reveal different metabolite biosynthesis profiles between leaf buds and mature leaves in Ziziphus jujuba mill

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2021-06-15 DOI:10.1016/j.foodchem.2021.129005

Shipeng Li, Benliang Deng, Shan Tian, Mingxin Guo, Hongxia Liu, Xusheng Zhao

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引用次数: 43

Abstract

Jujube leaf is well known for its high nutritional value and medicinal benefits. However, a thorough and dynamic assessment of the metabolites present in jujube leaves is lacking. Here, the primary and secondary metabolites from purple leaf buds and green mature leaves were investigated using ultra-high-performance liquid chromatography/tandem mass spectrometry. A total of 778 metabolites were characterized and more than 700 compounds were reported for the first time. Analysis of differentially accumulated metabolites showed that the flavonoids were the major differential metabolites and determined the leaf coloration. The transcriptome data indicated that 20 flavonoid structural genes and three main types of flavonoid regulatory genes were significantly differentially expressed. Moreover, light had a significant influence on flavonoid accumulation. These results improve our understanding of metabolite accumulation and the molecular mechanisms of flavonoid biosynthesis in jujube leaf.

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代谢和转录组学分析揭示了酸枣芽叶和成熟叶代谢物合成谱的差异

红枣叶以其高营养价值和药用价值而闻名。然而，缺乏对枣叶中代谢物的全面和动态评估。本研究采用超高效液相色谱/串联质谱法对紫色叶芽和绿色成熟叶的初级和次级代谢物进行了研究。共鉴定了778种代谢物，其中700多种化合物为首次报道。差异代谢物分析表明，黄酮类化合物是主要的差异代谢物，并决定了叶片的颜色。转录组数据显示，20个类黄酮结构基因和3种主要类黄酮调控基因的表达存在显著差异。光照对黄酮类化合物积累有显著影响。这些结果提高了我们对枣叶代谢产物积累和类黄酮生物合成分子机制的认识。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.