False positive diagnosis of meningococcal infection by the IS1106 PCR ELISA

IF 2.2 4区生物学 Q3 MICROBIOLOGY

Fems Microbiology Letters Pub Date : 2006-01-17 DOI:10.1111/j.1574-6968.1998.tb13001.x

Ray Borrow, Malcolm Guiver, Francesca Sadler, Edward B Kaczmarski, Andrew J Fox

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引用次数: 31

Abstract

At a time when optimal case ascertainment for meningococcal infection is a high priority, the need for non-culture case confirmation, in particular by DNA amplification, is seen as being of vital importance to assist contact management and cluster recognition. A solution hybridisation assay with colorimetric microtitre plate detection (polymerase chain reaction-enzyme-linked immunosorbent assay (PCR ELISA)) has been developed using the multicopy insertion sequence IS1106 which had reportedly achieved a specificity of 100% and was described as being meningococcal specific. This PCR ELISA assay was evaluated on specimens from over 5000 patients at the national Meningococcal Reference Unit (MRU) between late 1995 and early 1997 and was found to be highly sensitive. Insertion sequences, however, are genetically mobile with the ability to spread between species and even genera. During the evaluation period of the IS1106 PCR ELISA a number of false positives proved to be caused by organisms other than N. meningitidis were recorded resulting in the withdrawal of this assay as a front line screening assay for routine confirmation of meningococcal infection.

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IS1106 PCR ELISA对脑膜炎球菌感染的假阳性诊断

在对脑膜炎球菌感染进行最佳病例确定是高度优先事项的时候，需要对非培养病例进行确认，特别是通过DNA扩增进行确认，被视为对协助接触者管理和群集识别至关重要。利用多拷贝插入序列IS1106，采用比色微滴板检测(聚合酶链反应-酶联免疫吸附试验(PCR ELISA))开发了一种溶液杂交试验，据报道该方法的特异性为100%，并被描述为脑膜炎球菌特异性。1995年底至1997年初，在国家脑膜炎球菌参考单位(MRU)对5000多名患者的标本进行了PCR ELISA检测，结果发现高度敏感。然而，插入序列在基因上是可移动的，具有在物种甚至属之间传播的能力。在IS1106 PCR ELISA的评估期间，记录了许多被证明是由脑膜炎奈瑟菌以外的生物引起的假阳性，导致该检测不再作为常规确认脑膜炎球菌感染的一线筛查检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fems Microbiology Letters 生物-微生物学

CiteScore

4.30

自引率

0.00%

发文量

112

审稿时长

1.9 months

期刊介绍： FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.