Bioassay-guided preparation of antioxidant, anti-inflammatory active fraction from crabapples (Malus prunifolia (Willd.) Borkh.)

IF 10.4 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2023-04-16 Epub Date: 2022-11-26 DOI:10.1016/j.foodchem.2022.135091

Munire Abuduaini , Jun Li , Jing Hui Ruan , Yong Xin Zhao , Maiwulanjiang Maitinuer , Haji Akber Aisa

{"title":"Bioassay-guided preparation of antioxidant, anti-inflammatory active fraction from crabapples (Malus prunifolia (Willd.) Borkh.)","authors":"Munire Abuduaini , Jun Li , Jing Hui Ruan , Yong Xin Zhao , Maiwulanjiang Maitinuer , Haji Akber Aisa","doi":"10.1016/j.foodchem.2022.135091","DOIUrl":null,"url":null,"abstract":"<div>The aim of current study was to optimize the extraction process and purification of main components (MC) to obtain high antioxidant, anti-inflammatory effective fractions from crabapple (Malus prunifolia (Willd.) Borkh.). The effects of three variables including ethanol concentration A1, solid-liquid ratio A2, extraction temperature A3 were investigated and optimized by response surface methodology (RSM) coupled with Box-Behnken design (BBD). The adsorption/desorption characteristics of MC on the five types of macroporous resins were investigated. According to batch adsorption test, HPD-300 resins were selected for kinetics. The adsorption mechanism showed that the process was appropriate by pseudo-second-order kinetics model, and purification parameters of MC were optimized through adsorption/desorption experiments with the column packed by HPD-300 resin. The effective fractions were obviously superior to other fractions according to DPPH, ABTS, COX-2 and 15-LOX radical scavenging. This work implies that the purified active fraction with high contents of antioxidants and anti-inflammatory compounds from crabapple might be potential source for natural products and food industries.</div>","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"406 ","pages":"Article 135091"},"PeriodicalIF":10.4000,"publicationDate":"2023-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0308814622030539","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/11/26 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}

引用次数: 0

Abstract

The aim of current study was to optimize the extraction process and purification of main components (MC) to obtain high antioxidant, anti-inflammatory effective fractions from crabapple (Malus prunifolia (Willd.) Borkh.). The effects of three variables including ethanol concentration A₁, solid-liquid ratio A₂, extraction temperature A₃ were investigated and optimized by response surface methodology (RSM) coupled with Box-Behnken design (BBD). The adsorption/desorption characteristics of MC on the five types of macroporous resins were investigated. According to batch adsorption test, HPD-300 resins were selected for kinetics. The adsorption mechanism showed that the process was appropriate by pseudo-second-order kinetics model, and purification parameters of MC were optimized through adsorption/desorption experiments with the column packed by HPD-300 resin. The effective fractions were obviously superior to other fractions according to DPPH, ABTS, COX-2 and 15-LOX radical scavenging. This work implies that the purified active fraction with high contents of antioxidants and anti-inflammatory compounds from crabapple might be potential source for natural products and food industries.

Abstract Image

查看原文本刊更多论文

生物测定法制备海棠抗氧化、抗炎活性组分Borkh)。

本研究旨在优化海棠中主要成分(MC)的提取工艺和纯化，以获得高抗氧化、抗炎活性成分。Borkh)。采用响应面法(RSM)结合Box-Behnken设计(BBD)对乙醇浓度A1、料液比A2、提取温度A3 3个变量的影响进行了考察和优化。研究了MC在5种大孔树脂上的吸附/解吸特性。通过间歇吸附试验，选择HPD-300树脂进行动力学研究。拟二级吸附动力学模型表明，该吸附过程是适宜的，并通过HPD-300树脂填充柱的吸附/解吸实验优化了MC的纯化参数。有效部位对DPPH、ABTS、COX-2和15-LOX自由基的清除能力明显优于其他部位。研究结果表明，海棠中抗氧化、抗炎活性成分含量高，具有开发天然产品和食品工业的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.