Portable Biosensor with Bimetallic Metal–Organic Frameworks for Visual Detection and Elimination of Bacteria

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2023-08-23 DOI:10.1021/acs.analchem.3c02841

Yuting Shang, Gaowa Xing, Haifeng Lin, Shulang Chen, Tianze Xie and Jin-Ming Lin*,

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引用次数: 1

Abstract

A multifunctional platform that meets the demands of both bacterial detection and elimination is urgently needed because of their harm to human health. Herein, a “sense-and-treat” biosensor was developed by using immunomagnetic beads (IMBs) and AgPt nanoparticle-decorated PCN-223-Fe (AgPt/PCN-223-Fe, PCN stands for porous coordination network) metal–organic frameworks (MOFs). The synthesized AgPt/PCN-223-Fe not only exhibited excellent peroxidase-like activity but also could efficiently kill bacteria under near infrared (NIR) irradiation. This biosensor enabled the colorimetric detection of E. coli O157:H7 in the range of 10³–10⁸ CFU/mL with a limit of detection of 276 CFU/mL, accompanied with high selectivity, good reproducibility, and wide applicability in diverse real samples. Furthermore, the biosensor possessed a highly effective antibacterial rate of 99.94% against E. coli O157:H7 under 808 nm light irradiation for 20 min. This strategy can provide a reference for the design of novel versatile biosensors for bacterial discrimination and antibacterial applications.

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便携式双金属金属有机框架生物传感器的视觉检测和消除细菌

由于细菌对人体健康的危害，迫切需要一种既能检测又能消除细菌的多功能平台。本文采用免疫磁珠(IMBs)和AgPt纳米粒子修饰的PCN-223- fe (AgPt/PCN-223- fe, PCN代表多孔配位网络)金属有机框架(mof)开发了一种“感知和治疗”生物传感器。合成的AgPt/PCN-223-Fe不仅表现出优异的过氧化物酶样活性，而且在近红外(NIR)照射下能有效地杀死细菌。该传感器对大肠杆菌O157:H7的比色检测范围为103 ~ 108 CFU/mL，检出限为276 CFU/mL，具有选择性高、重现性好、适用范围广等特点。在808 nm光照射20 min下，该传感器对大肠杆菌O157:H7的抑菌率高达99.94%。该策略可为设计用于细菌鉴别和抗菌应用的新型多功能生物传感器提供参考。

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.