Novel dual immunochromatographic test strip based on double antibodies and biotin-streptavidin system for simultaneous sensitive detection of aflatoxin M1 and ochratoxin A in milk

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2022-05-01 DOI:10.1016/j.foodchem.2021.131682

Jiadi Sun , Miao Li , Fuguo Xing , Haiming Wang , Yinzhi Zhang , Xiulan Sun

{"title":"Novel dual immunochromatographic test strip based on double antibodies and biotin-streptavidin system for simultaneous sensitive detection of aflatoxin M1 and ochratoxin A in milk","authors":"Jiadi Sun , Miao Li , Fuguo Xing , Haiming Wang , Yinzhi Zhang , Xiulan Sun","doi":"10.1016/j.foodchem.2021.131682","DOIUrl":null,"url":null,"abstract":"<div><p>The coexistence of mycotoxins in agricultural products poses a serious threat to food safety. This study developed a dual immunochromatographic test strips (DICTS) method based on double antibodies labeled with time-resolved fluorescent microspheres (TRFM) to realize simultaneous rapid detection of aflatoxin M1 (AFM1) and ochratoxin A (OTA) in milk. As bridge antibody, the polyclonal antibody (pAb) was first conjugated with the TRFM and then with the monoclonal antibody (mAb). Meanwhile, a biotin-streptavidin system was introduced to replace the traditional goat anti-mouse Immunoglobulin G, thus providing a stable signal on the control line. After optimization, the detection limit of AFM1 and OTA in milk was respectively 0.018 and 0.036 ng/mL. The recoveries of intraassay and interassay experiments ranged from 89.65% to 103.99%. The accuracy, repeatability, and specificity of the developed TRFM-DICTS were estimated. The results of TRFM-DICTS showed a high consistency with those of the ultrahigh-performance liquid chromatography-tandem mass spectrometry.</p></div>","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"375 ","pages":"Article 131682"},"PeriodicalIF":8.5000,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"16","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0308814621026881","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}

引用次数: 16

Abstract

The coexistence of mycotoxins in agricultural products poses a serious threat to food safety. This study developed a dual immunochromatographic test strips (DICTS) method based on double antibodies labeled with time-resolved fluorescent microspheres (TRFM) to realize simultaneous rapid detection of aflatoxin M1 (AFM1) and ochratoxin A (OTA) in milk. As bridge antibody, the polyclonal antibody (pAb) was first conjugated with the TRFM and then with the monoclonal antibody (mAb). Meanwhile, a biotin-streptavidin system was introduced to replace the traditional goat anti-mouse Immunoglobulin G, thus providing a stable signal on the control line. After optimization, the detection limit of AFM1 and OTA in milk was respectively 0.018 and 0.036 ng/mL. The recoveries of intraassay and interassay experiments ranged from 89.65% to 103.99%. The accuracy, repeatability, and specificity of the developed TRFM-DICTS were estimated. The results of TRFM-DICTS showed a high consistency with those of the ultrahigh-performance liquid chromatography-tandem mass spectrometry.

查看原文本刊更多论文

基于双抗体和生物素-链亲和素系统的双免疫层析试纸条用于牛奶中黄曲霉毒素M1和赭曲霉毒素A的同时灵敏检测

真菌毒素在农产品中的共存对食品安全构成严重威胁。本研究开发了一种双检测试纸测试条(字典)方法基于双抗体标记与时间分辨荧光微球(TRFM)实现同时快速检测黄曲霉毒素M1 (AFM1)和赭曲霉毒素a (OTA)牛奶。多克隆抗体(pAb)作为桥接抗体，先与TRFM偶联，再与单克隆抗体(mAb)偶联。同时，引入生物素-链亲和素系统替代传统的山羊抗小鼠免疫球蛋白G，从而在控制线上提供稳定的信号。优化后,检出限AFM1和牛奶中OTA分别0.018和0.036 ng / mL。法内和法间加样回收率为89.65% ~ 103.99%。的准确性、可重复性和特异性发达TRFM-DICTS估计。trfs - dicts结果与超高效液相色谱-串联质谱分析结果具有较高的一致性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.