Haplotype variation at the transcription factor PpMYB10.1 contributes to color differences of flesh around core in peach

IF 4.6 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2026-10-27 DOI:10.1016/j.scienta.2026.115244

Xingyu Guo, Minghao Zhang, Zhengwen Ye, Yingjia Wang, Xiaofeng Yang, Changjie Xu, Zhongshan Gao, Mingshen Su, Huayu Liu, Jihong Du, Huijuan Zhou, Xianan Zhang, Jiabo Wang, Xiongwei Li

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引用次数: 0

Abstract

Red pigmentation around the fruit core is a common phenomenon in peaches, resulting from the accumulation of anthocyanins which is always associated with sour taste. However, minimizing this pigmentation is a key breeding objective for developing near-core-uncolored cultivars. To elucidate the mechanism underlying this trait, we compared the near-core-colored cultivar ‘JinXiu’ (JXiu) and the near-core-uncolored cultivar ‘JinXing’ (JXing). The anthocyanin content in JXiu was markedly higher than in JXing, with cyanidin-3-O-glucoside (C3G) identified as the predominant anthocyanin component, showing the greatest difference at the second ripening stage. Integrative analyses of transcriptomic and metabolomic data identified 7,186 differentially expressed genes (DEGs) across ripening stages. Weighted gene co-expression network analysis (WGCNA) revealed that the Magenta module was highly correlated (r > 0.9) with six polyphenols, including C3G. Key anthocyanin biosynthetic genes such as PpPAL2, PpCHS1-3, PpF3H1, PpANS, and Pp4CL6 showed strong co-expression and significantly higher expression in JXiu. Genome-wide variant analysis of JXiu F1 population further identified PpMYB10.1 (Prupe.3G163100) as the most divergent gene between red-cored and non-red-core accessions, with 38 SNPs forming a complete co-segregation across the analyzed individuals. Allele-specific expression analysis revealed predominantly monoallelic transcription of PpMYB10.1 in JXiu, with expression predominantly driven from the reference allele, suggesting the presence of allele-specific regulatory mechanisms. Several intragenic SNPs in PpMYB10.1 (Pp03:18222329, Pp03:18222365, and Pp03:18222368) were linked to allele-specific transcription occurring exclusively at stage r2, coinciding with maximal anthocyanin accumulation. These findings identify PpMYB10.1 as a strong candidate gene associated with anthocyanin accumulation and near-core coloration in peach and provide molecular breeding tool for this horticulturally important trait.

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转录因子PpMYB10.1的单倍型变异是桃果核周围果肉颜色差异的原因之一

果核周围的红色色素沉着是桃子的常见现象，这是由于花青素的积累造成的，而花青素总是与酸味联系在一起。然而，减少这种色素沉着是培育近核无色品种的关键育种目标。为了阐明这一性状的机制，我们比较了近核有色品种‘锦绣’（JXiu）和近核有色品种‘金星’（JXing）。JXiu的花青素含量显著高于JXing，其中花青素-3- o -葡萄糖苷（C3G）是主要的花青素成分，在第二成熟期差异最大。转录组学和代谢组学数据的综合分析确定了7,186个不同成熟阶段的差异表达基因（deg）。加权基因共表达网络分析（WGCNA）显示，Magenta模块与包括C3G在内的六种多酚高度相关（r > 0.9）。花青素生物合成关键基因PpPAL2、PpCHS1-3、PpF3H1、PpANS、Pp4CL6在JXiu中共表达较强，表达量显著增高。JXiu F1群体全基因组变异分析进一步发现PpMYB10.1 （Prupe.3G163100）是红核和非红核遗传源间差异最大的基因，38个snp在分析个体间形成了完整的共分离。等位基因特异性表达分析显示，PpMYB10.1在JXiu中以单等位基因转录为主，表达主要由参考等位基因驱动，提示存在等位基因特异性调控机制。PpMYB10.1的几个基因内snp （Pp03:18222329， Pp03:18222365和Pp03:18222368）与等位基因特异性转录相关，这些转录只发生在r2阶段，与最大的花青素积累相一致。这些发现表明PpMYB10.1是与桃花青素积累和近核着色相关的强候选基因，并为这一重要园艺性状的分子育种提供了工具。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.