Exogenous 6-benzylaminopurine application delays leaf yellowing in pak choi (Brassica rapa subsp. chinensis) during storage by regulating endogenous N⁶-benzyladenine metabolism and chlorophyll catabolism.

IF 4 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2026-10-27 DOI:10.1002/jsfa.71182

Liuli Song, Wenhui Hu, Jingyi Tang, Rui Li, Mingqian Zhang, Mengru Cao, Ruxue Liu, Ting Ke, Shuihe Wang, Haibo Luo

{"title":"Exogenous 6-benzylaminopurine application delays leaf yellowing in pak choi (Brassica rapa subsp. chinensis) during storage by regulating endogenous N6-benzyladenine metabolism and chlorophyll catabolism.","authors":"Liuli Song, Wenhui Hu, Jingyi Tang, Rui Li, Mingqian Zhang, Mengru Cao, Ruxue Liu, Ting Ke, Shuihe Wang, Haibo Luo","doi":"10.1002/jsfa.71182","DOIUrl":null,"url":null,"abstract":"Background: Postharvest yellowing of pak choi is a critical issue affecting its commercial quality. This study investigated the mechanism by which 6-benzylaminopurine (6-BA), a synthetic form of the cytokinin benzyladenine (BA), regulates endogenous cytokinin (CK) metabolism and the signal transduction involved in the yellowing of pak choi during storage at 20 °C.Results: Treatment with 6-BA inhibited the expression of the key chlorophyll catabolic genes BcCLH1 and BcSGR1, retarded chlorophyll breakdown, and eventually mitigated leaf yellowing. The treatment also maintained a high level of BA (the predominant CK in pak choi), enhanced the activity of cytokinin oxidase/dehydrogenase (CKX) and increased the transcript abundance of the associated genes BcCKX3/6/7, and upregulated the expression of the N-glycosylation-related UDP glycosyltransferase (UGT) genes BcUGT75D1/76C2/89A2. These changes accelerated BA degradation and inactivation, contributing to the maintenance of BA homeostasis. This relationship was also associated with the maintenance of greater expression of genes involved in CK transport (BcPUP1/5/9/13 and BcENT3) and signal transduction (BcAHK2/4, BcAHP3, and type A and B Arabidopsis response regulators (ARRs) BcARR4/6/7/9/15 and BcARR11/12/14/18, respectively) in 6-BA-treated pak choi.Conclusion: These findings suggest that the protective effects of 6-BA treatment against chlorophyll degradation may be attributed to elevated BA levels, enhanced signal transduction capacity, accelerated BA degradation and inactivation, and feedback regulation between type A and B BcARRs. The findings identify a candidate regulatory pathway and key regulatory genes involved in the CK-mediated delay of pak choi leaf yellowing. © 2026 Society of Chemical Industry.","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":4.0000,"publicationDate":"2026-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Science of Food and Agriculture","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1002/jsfa.71182","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Postharvest yellowing of pak choi is a critical issue affecting its commercial quality. This study investigated the mechanism by which 6-benzylaminopurine (6-BA), a synthetic form of the cytokinin benzyladenine (BA), regulates endogenous cytokinin (CK) metabolism and the signal transduction involved in the yellowing of pak choi during storage at 20 °C.

Results: Treatment with 6-BA inhibited the expression of the key chlorophyll catabolic genes BcCLH1 and BcSGR1, retarded chlorophyll breakdown, and eventually mitigated leaf yellowing. The treatment also maintained a high level of BA (the predominant CK in pak choi), enhanced the activity of cytokinin oxidase/dehydrogenase (CKX) and increased the transcript abundance of the associated genes BcCKX3/6/7, and upregulated the expression of the N-glycosylation-related UDP glycosyltransferase (UGT) genes BcUGT75D1/76C2/89A2. These changes accelerated BA degradation and inactivation, contributing to the maintenance of BA homeostasis. This relationship was also associated with the maintenance of greater expression of genes involved in CK transport (BcPUP1/5/9/13 and BcENT3) and signal transduction (BcAHK2/4, BcAHP3, and type A and B Arabidopsis response regulators (ARRs) BcARR4/6/7/9/15 and BcARR11/12/14/18, respectively) in 6-BA-treated pak choi.

Conclusion: These findings suggest that the protective effects of 6-BA treatment against chlorophyll degradation may be attributed to elevated BA levels, enhanced signal transduction capacity, accelerated BA degradation and inactivation, and feedback regulation between type A and B BcARRs. The findings identify a candidate regulatory pathway and key regulatory genes involved in the CK-mediated delay of pak choi leaf yellowing. © 2026 Society of Chemical Industry.

查看原文本刊更多论文

外源6-苄基氨基嘌呤延缓白菜叶片黄化。通过调节内源性n6 -苄基腺苷代谢和叶绿素分解代谢。

背景：小白菜采后发黄是影响其商品品质的关键问题。本研究探讨了细胞分裂素苄基ladenine （BA）的合成形式6-苄基氨基嘌呤（6-BA）调控内源性细胞分裂素（CK）代谢和信号转导参与小白菜20℃贮藏变黄的机制。结果：6-BA抑制了关键叶绿素分解代谢基因BcCLH1和BcSGR1的表达，延缓了叶绿素分解，最终减轻了叶片黄变。该处理还维持了高水平的BA（白菜中主要的CK），提高了细胞分裂素氧化酶/脱氢酶（CKX）的活性，增加了相关基因BcCKX3/6/7的转录丰富度，上调了n-糖基化相关的UDP糖基转移酶（UGT）基因BcUGT75D1/76C2/89A2的表达。这些变化加速了BA的降解和失活，有助于维持BA的稳态。这种关系还与6- ba处理的小白菜中参与CK运输（BcPUP1/5/9/13和BcENT3）和信号转导（BcAHK2/4、BcAHP3以及A型和B型拟南芥应答调节因子BcARR4/6/7/9/15和BcARR11/12/14/18）的基因表达增加有关。结论：6-BA处理对叶绿素降解的保护作用可能与提高BA水平、增强信号转导能力、加速BA降解和失活以及A型和B型bcarr之间的反馈调节有关。研究结果确定了ck介导的白菜叶黄变延迟的候选调控途径和关键调控基因。©2026化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.