Saponin conjugation improves p-CRISPR delivery by polyethylenimine modified carbon dots.

IF 2.5 4区工程技术 Q3 PHARMACOLOGY & PHARMACY

Bioimpacts Pub Date : 2026-08-19 eCollection Date: 2026-01-01 DOI:10.34172/bi.33272

Ali Hamad Abd Kelkawi, Seyede Mahtab Kamrani Mousavi, Akbar Hasanzadeh, Sara Saeedi, Behjat Kheiri Yeganeh Azar, Babak Kamali, Seyyed Emad Hooshmand, Mohadese Rastgoo Korande, Michael R Hamblin, Jawdat N Gaaib, Mahdi Karimi

{"title":"Saponin conjugation improves p-CRISPR delivery by polyethylenimine modified carbon dots.","authors":"Ali Hamad Abd Kelkawi, Seyede Mahtab Kamrani Mousavi, Akbar Hasanzadeh, Sara Saeedi, Behjat Kheiri Yeganeh Azar, Babak Kamali, Seyyed Emad Hooshmand, Mohadese Rastgoo Korande, Michael R Hamblin, Jawdat N Gaaib, Mahdi Karimi","doi":"10.34172/bi.33272","DOIUrl":null,"url":null,"abstract":"Introduction: Non-toxic and efficient delivery platforms are important for gene editing applications. In this report, the preparation of saponin-conjugated low molecular weight polyethyleneimine (PEI1.8kDa) modified carbon dots (CD-PEI-Sap) is described.Methods: Polyethyleneimine modified carbon dots (CD-PEI1.8kDa), were synthesized by one-step microwave-mediated pyrolysis of citric acid (CA) and PEI1.8kDa to produce non-toxic fluorescent particles. When saponin was added at this stage followed by microwave treatment a completely clear solution of CD-PEI-Sap was produced.Results: These 80 nm nanoparticle vectors not only transfected green fluorescent protein plasmid (p-GFP) (3 kb) with 60% expression in human embryonic kidney (HEK 293T) cells, and>60% in hard to transfect human breast adenocarcinoma cells (MCF7), but could also transfect a large clustered regularly interspaced short palindromic repeat plasmid (p-CRISPR) with 5-fold higher efficiency (50%) than the gold standard reagent, branched PEI MWt 25 kDa (PEI25kDa). Moreover, the CD-PEI-Sap system produced good transfection with a low concentration of p-CRISPR and in the presence of 30% fetal bovine serum (FBS).Conclusion: This system demonstrated efficient transfection and serum resistance, making it a promising tool for future gene editing research.","PeriodicalId":48614,"journal":{"name":"Bioimpacts","volume":"16 ","pages":"33272"},"PeriodicalIF":2.5000,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13677597/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioimpacts","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.34172/bi.33272","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction: Non-toxic and efficient delivery platforms are important for gene editing applications. In this report, the preparation of saponin-conjugated low molecular weight polyethyleneimine (PEI_1.8kDa) modified carbon dots (CD-PEI-Sap) is described.

Methods: Polyethyleneimine modified carbon dots (CD-PEI_1.8kDa), were synthesized by one-step microwave-mediated pyrolysis of citric acid (CA) and PEI_1.8kDa to produce non-toxic fluorescent particles. When saponin was added at this stage followed by microwave treatment a completely clear solution of CD-PEI-Sap was produced.

Results: These 80 nm nanoparticle vectors not only transfected green fluorescent protein plasmid (p-GFP) (3 kb) with 60% expression in human embryonic kidney (HEK 293T) cells, and>60% in hard to transfect human breast adenocarcinoma cells (MCF7), but could also transfect a large clustered regularly interspaced short palindromic repeat plasmid (p-CRISPR) with 5-fold higher efficiency (50%) than the gold standard reagent, branched PEI MWt 25 kDa (PEI_25kDa). Moreover, the CD-PEI-Sap system produced good transfection with a low concentration of p-CRISPR and in the presence of 30% fetal bovine serum (FBS).

Conclusion: This system demonstrated efficient transfection and serum resistance, making it a promising tool for future gene editing research.

查看原文本刊更多论文

皂素偶联改善聚乙烯亚胺修饰碳点的p-CRISPR传递。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Bioimpacts Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

4.80

自引率

7.70%

发文量

审稿时长

5 weeks

期刊介绍： BioImpacts (BI) is a peer-reviewed multidisciplinary international journal, covering original research articles, reviews, commentaries, hypotheses, methodologies, and visions/reflections dealing with all aspects of biological and biomedical researches at molecular, cellular, functional and translational dimensions.