Ultra-High Peak Capacity Exceeding 5 Peaks per Second Enabled by LC×LC-SLIM-qTOF-MS for the Analysis of Complex Samples

IF 7.3 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2026-10-20 DOI:10.1021/acs.analchem.6c03659

Priscilla Nhan,Cedric Thom,Sven W. Meckelmann,Oliver J. Schmitz

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Abstract

Comprehensive two-dimensional chromatography substantially enhances separation performance prior to mass spectrometric detection compared to conventional one-dimensional approaches. This results in (i) cleaner and more readily interpretable mass spectra and (ii) reduced ion suppression in atmospheric pressure ionization sources. To further increase separation performance within a reasonable total analysis time, comprehensive LC×LC was coupled to a SLIM-IM-qTOF-MS system for the analysis of complex herbal liqueur samples. To facilitate the processing of multidimensional data, a long modulation time of 4 min was applied. In addition, trap-based modulation was implemented to improve analyte focusing and optimize two-dimensional pre-separation. A peak capacity of LC×LCn = 201 was achieved for the LC×LC separation. Incorporating ion mobility as a third separation dimension further increased the total peak capacity. Total peak capacities of Ptotal(SLIM) = 16,683 and Ptotal(DTIMS) = 8643 were achieved for the SLIM and DTIMS coupling, respectively. The SLIM coupling therefore provided an approximately 1.9-fold higher total peak capacity than the DTIMS coupling, demonstrating its superior separation potential.

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每秒超过5个峰的超高峰值容量通过LC×LC-SLIM-qTOF-MS支持复杂样品的分析

与传统的一维方法相比，全面的二维色谱法大大提高了质谱检测之前的分离性能。这导致(i)更清洁和更容易解释的质谱和（ii）减少大气压力电离源中的离子抑制。为了在合理的总分析时间内进一步提高分离性能，将综合LC×LC与SLIM-IM-qTOF-MS系统耦合，用于复杂草药利口酒样品的分析。为了方便处理多维数据，采用了4 min的长调制时间。此外，实现了基于陷阱的调制，以提高分析物聚焦和优化二维预分离。LC×LC分离的峰值容量为LC×LCn = 201。结合离子迁移率作为第三个分离维度进一步增加了总峰值容量。在SLIM和DTIMS耦合下，Ptotal（SLIM）和Ptotal（DTIMS）的总峰值容量分别为16683和8643。因此，SLIM耦合提供的总峰值容量比DTIMS耦合高约1.9倍，显示出其优越的分离潜力。

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.