Isolation and Characteristics of the Novel Proteinaceous Inhibitor against Pancreatic α-Amylase from White Kidney Bean ( Phaseolus vulgaris )

IF 6.7 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-16 DOI:10.1021/acs.jafc.6c12164

Yiting Wang,Jingyi Lu,Jialei Chu,Guangrong Huang,Wenwei Chen,Zhenbao Jia,Fei Tao

{"title":"Isolation and Characteristics\nof the Novel Proteinaceous\nInhibitor against Pancreatic α-Amylase from White Kidney\nBean (\n Phaseolus vulgaris\n )","authors":"Yiting Wang,Jingyi Lu,Jialei Chu,Guangrong Huang,Wenwei Chen,Zhenbao Jia,Fei Tao","doi":"10.1021/acs.jafc.6c12164","DOIUrl":null,"url":null,"abstract":"Abstract A novel proteinaceous α-amylase inhibitor was isolated from white kidney bean (Phaseolus vulgaris) by ammonium sulfate fractionation followed by DEAE-Sepharose Fast Flow and HiTrap DEAE-Sepharose Fast Flow chromatography. The purified inhibitor was identified as annexin (UniProt accession: V7BSM8) by HPLC-MS/MS, with a molecular mass of 35.97 kDa determined by MALDI-TOF MS. Annexin inhibited pancreatic α-amylase in a concentration-dependent manner, with an IC50 of 8.81 μM. The kinetic results were consistent with a noncompetitive inhibition pattern. Intrinsic fluorescence spectroscopy indicated that annexin binding to α-amylase induced static fluorescence quenching and conformational changes in the enzyme. Molecular dynamics simulations supported formation of a stable α-amylase–annexin complex, with electrostatic and van der Waals interactions as major energetic contributors. These findings identify annexin as a previously unrecognized α-amylase inhibitor from P. vulgaris with potential for modulating starch digestion and postprandial glycemic responses.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"12 1","pages":""},"PeriodicalIF":6.7000,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Agricultural and Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1021/acs.jafc.6c12164","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

Abstract A novel proteinaceous α-amylase inhibitor was isolated from white kidney bean (Phaseolus vulgaris) by ammonium sulfate fractionation followed by DEAE-Sepharose Fast Flow and HiTrap DEAE-Sepharose Fast Flow chromatography. The purified inhibitor was identified as annexin (UniProt accession: V7BSM8) by HPLC-MS/MS, with a molecular mass of 35.97 kDa determined by MALDI-TOF MS. Annexin inhibited pancreatic α-amylase in a concentration-dependent manner, with an IC50 of 8.81 μM. The kinetic results were consistent with a noncompetitive inhibition pattern. Intrinsic fluorescence spectroscopy indicated that annexin binding to α-amylase induced static fluorescence quenching and conformational changes in the enzyme. Molecular dynamics simulations supported formation of a stable α-amylase–annexin complex, with electrostatic and van der Waals interactions as major energetic contributors. These findings identify annexin as a previously unrecognized α-amylase inhibitor from P. vulgaris with potential for modulating starch digestion and postprandial glycemic responses.

查看原文本刊更多论文

白菜豆胰α-淀粉酶蛋白抑制剂的分离及特性研究

摘要采用硫酸铵分馏- DEAE-Sepharose Fast Flow - HiTrap DEAE-Sepharose Fast Flow色谱法从白芸豆（Phaseolus vulgaris）中分离得到一种新型的蛋白类α-淀粉酶抑制剂。纯化后的抑制剂经HPLC-MS/MS鉴定为膜联蛋白（UniProt登录号：V7BSM8）， MALDI-TOF MS测定其分子量为35.97 kDa，膜联蛋白对胰腺α-淀粉酶具有浓度依赖性，IC50为8.81 μM。动力学结果与非竞争性抑制模式一致。本征荧光光谱表明，膜联蛋白与α-淀粉酶结合引起α-淀粉酶的静态荧光猝灭和构象变化。分子动力学模拟支持稳定α-淀粉酶-膜联蛋白复合物的形成，静电和范德华相互作用是主要的能量贡献者。这些发现表明，膜联蛋白是一种以前未被发现的α-淀粉酶抑制剂，具有调节淀粉消化和餐后血糖反应的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.