HZP66 and ERF122 are involved in flavonoid metabolism of kiwifruit regulated by ethylene and 1-MCP

IF 4.6 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2026-10-01 Epub Date: 2026-10-17 DOI:10.1016/j.scienta.2026.115250

Hao Ni, Han Dai, Yi-hang Zhang, Zi-yi Wei, Yu-cheng Zhang, Hao-miao Ding, Wei Chen, Zhen-feng Yang, Wei Wu

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引用次数: 0

Abstract

Flavonoids are the core nutritional components and key indicators for quality evaluation of kiwifruit. Ethylene and 1-methylcyclopropene (1-MCP) treatments, as common postharvest regulation technologies, have been proven to promote flavonoid accumulation, but their underlying molecular mechanisms remain unclear. To elucidate the transcriptional regulatory mechanism of flavonoid metabolism in postharvest kiwifruit, this study used ‘Hongyang’ kiwifruit as material, and established ethylene, 1-MCP and control groups. A systematic analysis was conducted by integrating physiological assays, transcriptome analysis, dual-luciferase assay and electrophoretic mobility shift assay (EMSA). A total of 285 differentially expressed genes were screened, and 10 potential transcription factors were identified. Among them, ERF122 and HZP66 could directly activate the promoters of AcPAL1 and AcPAL2 respectively, the key structural genes involved in flavonoid biosynthesis. These findings refine the molecular regulatory network of flavonoid metabolism in kiwifruit, and also provide theoretical support and molecular targets for kiwifruit breeding and postharvest quality regulation.

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HZP66和ERF122参与了乙烯和1-MCP调控的猕猴桃类黄酮代谢

黄酮类化合物是猕猴桃的核心营养成分，也是猕猴桃品质评价的关键指标。乙烯和1-甲基环丙烯（1-MCP）处理作为常见的采后调控技术，已被证明可促进黄酮类化合物的积累，但其潜在的分子机制尚不清楚。为阐明猕猴桃采后类黄酮代谢的转录调控机制，本研究以‘红阳’猕猴桃为材料，分别建立乙烯、1-MCP和对照组。采用生理分析、转录组分析、双荧光素酶分析和电泳迁移转移分析（EMSA）进行系统分析。共筛选出285个差异表达基因，鉴定出10个潜在的转录因子。其中ERF122和HZP66分别能直接激活参与类黄酮生物合成的关键结构基因AcPAL1和AcPAL2的启动子。这些发现完善了猕猴桃类黄酮代谢的分子调控网络，也为猕猴桃育种和采后品质调控提供了理论支持和分子靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.