The transcription factor VvMYB308 positively regulates salt stress tolerance in Vitis vinifera

IF 4.6 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2026-10-01 Epub Date: 2026-10-13 DOI:10.1016/j.scienta.2026.115234

Man Zhang, Ling Su, Yingchun Chen, Zhihua Song, Tingting Du, Na Li, Xinbin Jiang, Hongyan Cao, Dong Meng, Qing Yang, Bo Li, Lei Gong

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Abstract

Soil salinization significantly limits agricultural yields. The results indicated that ‘Kyoho’ as the most salt-tolerant cultivar among four tested grape varieties. Moreover, transcriptome analysis showed that VvMYB308 and VvCHI2(chalcone isomerase 2) were key genes associated with flavonoid synthesis and respond to salt stress in grapes. The physiological analysis indicated substantially increased quercitrin levels in the salt-tolerant ‘Kyoho’ variety under salt stress. Subcellular localization analysis showed that VvMYB308 resided in the nucleus and interacted with the VvCHI2 promoter. Similarly, transient transformation assays revealed that VvMYB308 positively modulated salt tolerance in grape tissue-cultured seedlings. This study indicated that the VvMYB308-VvCHI2 pathway modulated grape salt tolerance by promoting quercitrin synthesis. These findings provide theoretical evidence for the breeding of salt-tolerant grape varieties and their cultivation in saline‒alkali soils.

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转录因子VvMYB308正调控葡萄耐盐性

土壤盐渍化严重限制了农业产量。结果表明，“京侯”是4个葡萄品种中最耐盐的品种。转录组分析表明，VvMYB308和VvCHI2（查尔酮异构酶2）是葡萄类黄酮合成和响应盐胁迫的关键基因。生理分析表明，盐胁迫下耐盐品种Kyoho的槲皮素水平显著升高。亚细胞定位分析表明，VvMYB308位于细胞核内，并与VvCHI2启动子相互作用。同样，瞬时转化实验显示，VvMYB308正调节葡萄组培苗的耐盐性。本研究表明，VvMYB308-VvCHI2通路通过促进槲皮素合成来调节葡萄的耐盐性。这些研究结果为耐盐葡萄品种的选育和盐碱地栽培提供了理论依据。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.