Establishing efficient in vitro tissue culture systems for three types of explants of Gypsophila paniculata

IF 4.6 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2026-10-01 Epub Date: 2026-10-14 DOI:10.1016/j.scienta.2026.115205

Mingdong Ran, Yue Xu, Hongjia Zhang, Huameng Huang, Kang Luo, Chao Song, Muhammad Sajjad, Lijuan Ding, Xiaowei Yu, Guoping Zhang, Yun Zheng

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引用次数: 0

Abstract

Gypsophila paniculata, commonly known as baby’s breath, is a perennial herbaceous plant belonging to the genus Gypsophila within the Caryophyllaceae family. It encompasses two cultivated varieties: single-petaled and double-petaled. Double-petaled variants exhibit densely layered petals, conferring high ornamental value and physiological significance. However, double-petaled G. paniculata suffers from severe sterility and lacks a stable and efficient regeneration system. This study developed efficient in vitro tissue culture regeneration protocols for three explants, i.e., floral buds, leaves, and stem segments from a double-petaled cultivar of G. paniculata, ‘YX-4’. We found that different types of explants need different plant growth regulators. Unopened floral buds achieved a high shoot induction rate of 75%, and leaf explants achieved the highest callus induction rate of 76%. Leaf-derived callus exhibits the highest shoot induction rate of 50%. These optimized systems provide an important foundation for transgenic research and commercial propagation of double-petaled G. paniculata.

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建立三种吉思兰外植体的高效离体组织培养体系

吉思兰，俗称婴儿呼吸，是一种多年生草本植物，属于石竹科吉思兰属。它包括两种栽培品种：单花瓣和双花瓣。重瓣变种具有密集的层状花瓣，具有很高的观赏价值和生理意义。然而，重瓣金银花存在严重的不育性，且缺乏稳定高效的再生体系。本研究以双瓣金莲（G. paniculata, y -4）为材料，对花蕾、叶片和茎段进行了高效的离体组织培养再生。我们发现不同类型的外植体需要不同的植物生长调节剂。未开放的花芽诱导率最高，为75%，叶片外植体愈伤组织诱导率最高，为76%。叶片愈伤组织诱导率最高，为50%。这些优化的体系为双瓣金穗的转基因研究和商业化繁殖提供了重要的基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.