A novel temperature-responsive biphasic deep eutectic solvent for one-step extraction and selective enrichment of multi-polarity bioactives from green tea

IF 10.4 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2026-12-01 Epub Date: 2026-09-26 DOI:10.1016/j.foodchem.2026.151331

Xuerong Yang, Ying Shao, Mengtao Jin, Wentao Ye, Zaifa Pan, Zuguang Li, Jinsong Liu

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引用次数: 0

Abstract

Green tea contains bioactive components with varying polarities. Single deep eutectic solvents (DESs) struggle to achieve simultaneous extraction of different polarity targets.

This study developed a temperature-responsive biphasic DES (TRBDES) system for the selective extraction of six bioactive substances with distinct polarities from green tea. The system exhibited reversible biphasic-monophasic transition via temperature modulation. Under optimal conditions, the total extraction efficiency reached 158.24 ± 6.30 mg/g, significantly higher than conventional methods. Hydrophilic (e.g., EGCG) and hydrophobic (e.g., chlorophyll) components were almost completely partitioned into the lower and upper phases, respectively, while caffeine was distributed between both phases. Notably, the TRBDES extracts exhibited superior antioxidant activity (4.4-fold increase over ethanol extraction) and low melting points, confirming structural formation and functionality. Density functional theory calculations elucidated the interaction mechanisms. Demonstrating environmental friendliness, operational simplicity, and strong cell fiber structure disruption capability, this system provides a novel strategy for multi-polarity bioactive substance extraction.

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一种新型温度响应型双相深度共晶溶剂，用于一步萃取和选择性富集绿茶多极性生物活性物质

绿茶含有不同极性的生物活性成分。单一的深共晶溶剂（DESs）难以同时提取不同极性的目标。本研究建立了一种温度响应双相DES （TRBDES）系统，用于从绿茶中选择性提取六种极性不同的生物活性物质。通过温度调制，系统表现出可逆的双相-单相转变。在最佳条件下，总提取率达到158.24±6.30 mg/g，显著高于常规方法。亲水成分（如EGCG）和疏水成分（如叶绿素）几乎完全分别被划分为上下相，而咖啡因则分布在两相之间。值得注意的是，TRBDES提取物具有优异的抗氧化活性（比乙醇提取物提高4.4倍）和低熔点，证实了结构形成和功能。密度泛函理论计算阐明了相互作用机理。该系统具有环境友好、操作简单、破坏细胞纤维结构能力强等特点，为多极性生物活性物质的提取提供了新的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.