Niosome-encapsulated hesperidin accelerates antioxidant defense and TRPM2 protein expression to restore cognitive function in a rat model of depression

IF 4.5 3区医学 Q2 NEUROSCIENCES

Brain Research Bulletin Pub Date : 2026-10-15 Epub Date: 2026-09-25 DOI:10.1016/j.brainresbull.2026.112137

Mahsa Otarkhani, Homeira Hatami, Yousef panahi, Sama Yavari, Somayeh Hatami, Maryam Azami, Ali Eslami

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引用次数: 0

Abstract

Aims

Hesperidin is a potent antioxidant; however, its therapeutic efficacy is hindered by limited bioavailability. Furthermore, the role of TRPM2 in oxidative stress-induced depression remains to be elucidated. This study investigated the therapeutic potential of niosomal hesperidin in a reserpine-induced depression rat model, focusing on learning and memory performance, oxidative stress markers, and TRPM2 protein expression.

Methods and materials

36 rats were randomly divided into six groups: Control, depression (Reserpine), free hesperidin, Niosomal hesperidin, treatment with hesperidin, and treatment with niosomal hesperidin. Reserpine 0.5 mg/kg was administered intraperitoneally for 14 days. Then, rats received hesperidin (20 mg/kg, 14 days) and niosomal hesperidin (20 mg/kg, 7 days). The novel object recognition and shuttle box tests were performed. Finally, glutathione peroxidase levels, serum superoxide dismutase, and western blotting for TRPM2 protein expression were measured.

Results

SOD activity and GPX levels increased in the reserpine-induced rats treated with hesperidin and niosome hesperidin (P < 0.05). TRPM2 protein expression decreased in niosome-hesperidin-treated groups compared to the depression group (P < 0.0001). The discrimination index increased in the reserpine-induced rats treated with niosome hesperidin compared to the depression group (P < 0.001). The total time spent in the white box increased in the shuttle box in the reserpine-induced rats treated with niosome hesperidin compared to the control group (P < 0.05).

Conclusion

Niosomal encapsulation accelerates cognitive recovery and modulates antioxidant capacity and TRPM2 protein expression. Thus, niosomal hesperidin represents a promising nanocarrier strategy for managing depression-related cognitive dysfunction.

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nioase包封橙皮苷加速抗氧化防御和TRPM2蛋白表达以恢复抑郁症大鼠模型的认知功能

目的：橙皮苷是一种有效的抗氧化剂；然而，其治疗效果受到有限的生物利用度的阻碍。此外，TRPM2在氧化应激诱导的抑郁症中的作用仍有待阐明。本研究探讨了niosomal橙皮苷在利血平诱导的抑郁症大鼠模型中的治疗潜力，重点关注学习和记忆表现、氧化应激标志物和TRPM2蛋白表达。方法与材料：36只大鼠随机分为对照组、抑制组（利血平）、游离橙皮苷组、橙皮苷组、橙皮苷组、橙皮苷组和橙皮苷组。利血平0.5mg/kg腹腔注射，连续14天。然后给大鼠注射橙皮苷（20mg/kg, 14 d）和niosomal橙皮苷（20mg/kg, 7 d）。进行了新型目标识别和穿梭箱试验。最后，测定谷胱甘肽过氧化物酶水平、血清超氧化物歧化酶水平和TRPM2蛋白表达的western blotting。结果：利血平诱导的大鼠经橙皮苷和Niosomal橙皮苷处理后，SOD活性和GPX水平升高。结论：Niosomal encapsulation加速认知恢复，调节抗氧化能力和TRPM2蛋白表达。因此，niosomal橙皮苷代表了一种治疗抑郁症相关认知功能障碍的有前途的纳米载体策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Brain Research Bulletin 医学-神经科学

CiteScore

6.90

自引率

2.60%

发文量

253

审稿时长

67 days

期刊介绍： The Brain Research Bulletin (BRB) aims to publish novel work that advances our knowledge of molecular and cellular mechanisms that underlie neural network properties associated with behavior, cognition and other brain functions during neurodevelopment and in the adult. Although clinical research is out of the Journal''s scope, the BRB also aims to publish translation research that provides insight into biological mechanisms and processes associated with neurodegeneration mechanisms, neurological diseases and neuropsychiatric disorders. The Journal is especially interested in research using novel methodologies, such as optogenetics, multielectrode array recordings and life imaging in wild-type and genetically-modified animal models, with the goal to advance our understanding of how neurons, glia and networks function in vivo.