Halotolerant Pseudomonas cedrina strains enhance salinity tolerance and fruit yield in strawberry through physiological responses and improved ion homeostasis

IF 4.6 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2026-09-01 Epub Date: 2026-09-21 DOI:10.1016/j.scienta.2026.115186

Shahriyar Einizadeh, Ali Akbar Shokouhian, Rasoul Azarmi

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引用次数: 0

Abstract

Salinity stress critically constrains strawberry (Fragaria × ananassa Duch.) production, particularly in regions affected by secondary salinization. This study evaluated the efficacy of two halotolerant plant growth-promoting rhizobacteria (PGPR), Pseudomonas cedrina SH_EI23 (SHA) and SH_EI24 (SHB), isolated from native halophyte rhizospheres in the Lake Urmia basin, Iran, in mitigating salinity effects on strawberry cv. Albion. Plants were grown under soil and soilless systems and exposed to 0, 30, and 60 mM NaCl. Salinity severely reduced vegetative growth, photosynthetic efficiency (Fv/Fm), and fruit yield, while increasing oxidative stress markers (MDA, electrolyte leakage) and Na⁺ accumulation. However, PGPR inoculation, particularly SHA, significantly ameliorated these adverse effects. Under 60 mM NaCl, SHA inoculation substantially increased fruit yield compared to non-inoculated controls, with a greater response observed in the soilless system. Inoculated plants maintained higher relative water content, stomatal conductance, and Fv/Fm, alongside enhanced proline and soluble carbohydrate accumulation. Antioxidant enzyme activities (CAT, SOD, POD) were significantly increased, while MDA content and electrolyte leakage decreased. PGPR also improved ionic homeostasis by increasing K⁺/Na⁺ ratios and increasing leaf N, P, Ca²⁺, and Mg²⁺ concentrations. Three-way ANOVA revealed that cultivation system did not significantly affect vegetative growth; however, the significant System × Inoculation interactions for key traits confirmed that PGPR efficacy was more pronounced in the soilless system. These findings indicate that halophyte-derived P. cedrina strains, particularly SHA, can enhance strawberry salinity tolerance through coordinated improvements in photosynthetic performance, osmotic adjustment, antioxidant defense, and ion homeostasis. Overall, the results suggest that native PGPR strains have strong potential as sustainable bio-inoculants for strawberry production in salt-affected regions. However, field validation across multiple cultivars and environments remains necessary before commercial application.

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耐盐木绿假单胞菌菌株通过生理反应和离子稳态的改善提高草莓的耐盐性和果实产量

盐度胁迫严重限制草莓（Fragaria × ananassa Duch.）的生产，特别是在受次生盐碱化影响的地区。研究了从伊朗乌尔米米亚湖盆地原生盐生植物根际分离得到的两种耐盐促生根瘤菌——绿假单胞菌SH_EI23 （SHA）和SH_EI24 （SHB）对草莓的抑盐作用。阿尔比恩。植物在土壤和无土系统下生长，并暴露于0、30和60 mM NaCl。盐度严重降低了营养生长、光合效率（Fv/Fm）和果实产量，同时增加了氧化应激标志物（MDA、电解质泄漏）和Na⁺的积累。然而，接种PGPR，特别是SHA，显著改善了这些不良反应。在60 mM NaCl条件下，与未接种对照相比，接种SHA显著提高了果实产量，在无土体系中观察到更大的响应。接种植株保持较高的相对含水量、气孔导度和Fv/Fm，同时增加脯氨酸和可溶性碳水化合物的积累。抗氧化酶活性（CAT、SOD、POD）显著升高，MDA含量和电解质泄漏降低。PGPR还通过提高K + /Na +的比例和叶片N、P、Ca 2 +和Mg 2 +的浓度来改善离子稳态。三因素方差分析表明，栽培制度对营养生长无显著影响；然而，系统 × 接种对关键性状的显著相互作用证实了PGPR在无土系统中的作用更为明显。这些研究结果表明，盐生植物衍生的P. cedrina菌株，特别是SHA，可以通过协调改善光合性能、渗透调节、抗氧化防御和离子稳态来提高草莓的耐盐性。综上所述，本地PGPR菌株具有很强的潜力，可以作为盐害地区草莓生产的可持续生物接种剂。然而，在商业应用之前，跨多种品种和环境的田间验证仍然是必要的。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.