Compositional Variability in Algerian Monocultivar Extra Virgin Olive Oils: A UPLC-DAD Approach to Fatty Acid Profiling Across Regions, Cultivars, and Seasons
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引用次数: 0
Abstract
This study reports a comprehensive assessment of the fatty acid composition in Algerian Monocultivar Extra Virgin Olive Oils (MEVOOs) using a fully validated, derivatization-free ultra-performance liquid chromatography coupled with diode-array detection (UPLC-DAD) method. A total of 45 MEVOO samples, collected from contrasting agroclimatic regions of Algeria, were analyzed over two consecutive harvest seasons to investigate the effects of cultivar, geographical origin, and seasonal variability. Oleic acid (C18:1 n-9) was the predominant fatty acid (43.53%–72.88%), confirming the generally favorable nutritional profile of Algerian MEVOOs. However, oils originating from arid regions exhibited notable deviations from International Olive Council (IOC) and European Union (EU) standards, particularly elevated stearic and linolenic acid contents, which are likely associated with thermal and water stress. The UPLC-DAD method demonstrated excellent selectivity, linearity (R2 ≥ 0.97), precision (RSD < 5%), accuracy (92%–105%), and low limits of detection and quantification. Multivariate statistical analyses highlighted strong biosynthetic trade-offs between saturated and monounsaturated fatty acids. These findings support the suitability of the UPLC-DAD method as a robust analytical tool for routine quality control and provide valuable insights for the development of region-specific quality benchmarks for Algerian olive oils.
期刊介绍：
The Journal of the American Oil Chemists’ Society (JAOCS) is an international peer-reviewed journal that publishes significant original scientific research and technological advances on fats, oils, oilseed proteins, and related materials through original research articles, invited reviews, short communications, and letters to the editor. We seek to publish reports that will significantly advance scientific understanding through hypothesis driven research, innovations, and important new information pertaining to analysis, properties, processing, products, and applications of these food and industrial resources. Breakthroughs in food science and technology, biotechnology (including genomics, biomechanisms, biocatalysis and bioprocessing), and industrial products and applications are particularly appropriate.
JAOCS also considers reports on the lipid composition of new, unique, and traditional sources of lipids that definitively address a research hypothesis and advances scientific understanding. However, the genus and species of the source must be verified by appropriate means of classification. In addition, the GPS location of the harvested materials and seed or vegetative samples should be deposited in an accredited germplasm repository. Compositional data suitable for Original Research Articles must embody replicated estimate of tissue constituents, such as oil, protein, carbohydrate, fatty acid, phospholipid, tocopherol, sterol, and carotenoid compositions. Other components unique to the specific plant or animal source may be reported. Furthermore, lipid composition papers should incorporate elements of yeartoyear, environmental, and/ or cultivar variations through use of appropriate statistical analyses.
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