A field-deployable microfluidic platform for point-of-care testing of aquaculture biosecurity.

IF 9.7 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

NPJ Science of Food Pub Date : 2026-07-14 DOI:10.1038/s41538-026-01003-2

Lei Xu, Fei Yao, Caroline Zhang, Xiaoyuan Wan, Chengyin Shi, Feng Shen

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引用次数: 0

Abstract

Infectious diseases persistently threaten aquaculture, particularly in shrimp farming, which urgently requires deployable diagnostic technologies for biosecurity management. However, current methods are limited by complex sample matrices, reliance on centralized laboratories, and delayed data feedback, making them unsuitable for on-site detection in decentralized settings. Here, we reported a deployable platform utilizing injection molded SlipChip microfluidic device that performs automated nucleic acid testing from shrimp tissues in aquaculture settings. The platform successfully detected Enterocytozoon hepatopenaei, white spot syndrome virus, highly lethal Vibrio parahaemolyticus, and decapod iridescent virus 1 as low as 200 copies/reaction within 35 min. The results of 103 shrimp samples on the platform were highly concordant with qPCR (κ = 0.957). Additionally, untrained farmers were able to independently carry out pathogen detection without complex manual operations. This platform enabled on-site testing for timely infection management, and holds promise for broader POCT applications in food safety.

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一种现场可部署的微流控平台，用于水产养殖生物安全的即时检测。

传染病持续威胁着水产养殖，特别是对虾养殖，这迫切需要可部署的诊断技术来进行生物安全管理。然而，目前的方法受到复杂的样品矩阵、依赖集中式实验室和延迟的数据反馈的限制，使其不适合分散设置的现场检测。在这里，我们报告了一个可展开的平台，利用注塑成型的SlipChip微流体装置，在水产养殖环境中对虾组织进行自动核酸检测。该平台在35分钟内成功检测出肝芽胞虫、白斑综合征病毒、高致死率的副溶血性弧菌和低至200拷贝/反应的十足虫虹彩病毒1型。平台上103份对虾样品的qPCR结果与qPCR结果高度吻合（κ = 0.957）。此外，未经培训的农民能够独立进行病原体检测，而无需复杂的人工操作。该平台使现场检测能够及时进行感染管理，并有望在食品安全方面更广泛地应用POCT。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

NPJ Science of Food FOOD SCIENCE & TECHNOLOGY-

CiteScore

7.50

自引率

1.60%

发文量

期刊介绍： npj Science of Food is an online-only and open access journal publishes high-quality, high-impact papers related to food safety, security, integrated production, processing and packaging, the changes and interactions of food components, and the influence on health and wellness properties of food. The journal will support fundamental studies that advance the science of food beyond the classic focus on processing, thereby addressing basic inquiries around food from the public and industry. It will also support research that might result in innovation of technologies and products that are public-friendly while promoting the United Nations sustainable development goals.