Elicitors derived from endophytic fungus Acremonium sp. enhance triterpenoid and polysaccharide production in the submerged cultivation of the medicinal mushroom Inonotus obliquus.

IF 4.3 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Bioprocess and Biosystems Engineering Pub Date : 2026-08-01 Epub Date: 2026-07-02 DOI:10.1007/s00449-026-03378-8

Ming Yang, Yaqi Shi, Yong Sun, Jiangsheng Zhou, Jihong Jiang

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Abstract

Triterpenoids and polysaccharides produced by Inonotus obliquus exhibit antitumor, antioxidant and hypoglycemic activities. Increasing the yields of triterpenoids and polysaccharides could effectively enhance the medicinal value of I. obliquus. In this study, the feasibility of improving the production of triterpenoids and polysaccharides by adding elicitors including cell wall debris (CW) and culture filtrate (CF) derived from endophytic fungus Acremonium sp. in the submerged cultivation of I. obliquus was investigated. The optimal concentrations and times for the addition of CW and CF were determined to be 60 µg/mL on day 3 and 10% v/v on day 1, respectively. The maximum total triterpenoid production of CW and CF groups increased by 19.38% and 49.81%, respectively, compared to the control. The maximum intracellular polysaccharide (IPS) production obtained with the CW and CF were 3.97 ± 0.24 and 4.95 ± 0.40 g/L, representing significant increases of 17.70% and 46.85% compared to the control. Additionally, the production of extracellular polysaccharides (EPS) was enhanced by 27.23% and 45.35% with the CW and CF, respectively, in comparison to the control. Furthermore, the transcription levels of the key genes involved in the biosynthesis of triterpenoids and polysaccharides were significantly upregulated in response to the elicitors. This study provides a novel approach for enhancing the bioactive metabolites production of medicinal basidiomycota by the elicitors derived from endophytes.

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从内生真菌Acremonium sp.提取的激发子提高了药用菌Inonotus obliquus潜水培养中三萜和多糖的产量。

斜树苗所产生的三萜和多糖具有抗肿瘤、抗氧化和降糖活性。提高三萜类和多糖的产率可以有效地提高斜刺草的药用价值。本研究研究了在水培养条件下，通过添加内生真菌Acremonium sp.的细胞壁碎片（CW）和培养滤液（CF）等激发子，提高斜叶菊（I. obliquus）三萜和多糖产量的可行性。第3天CW和CF的最佳添加浓度为60µg/mL，第1天添加量为10% v/v。CW组和CF组的最大总三萜产量分别比对照提高19.38%和49.81%。CW和CF的最大胞内多糖（IPS）产量分别为3.97±0.24和4.95±0.40 g/L，分别较对照显著提高了17.70%和46.85%。此外，与对照组相比，CW和CF分别增加了27.23%和45.35%的胞外多糖（EPS）的产量。此外，参与三萜类和多糖生物合成的关键基因的转录水平在激发子的作用下显著上调。本研究为利用内生菌衍生的激发子促进药用担子菌生物活性代谢产物的产生提供了一种新的途径。

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来源期刊

Bioprocess and Biosystems Engineering 工程技术-工程：化工

CiteScore

7.90

自引率

2.60%

发文量

147

审稿时长

2.6 months

期刊介绍： Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.