Preparation, characterization and evaluation of polysaccharides from Rosa roxburghii Tratt. fruit on ameliorating senior osteoporosis in rats induced by d-galactose and low calcium diet.

IF 4 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2026-09-01 Epub Date: 2026-06-23 DOI:10.1002/jsfa.70756

Yanfang Yan, Liangqun Li, Zhongsheng Luo, Tingfei Deng, Mei Zhou, Juan Yang, Qiji Li, Xiaosheng Yang, Lei Tang, Haitao Liu

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引用次数: 0

Abstract

Background: Senior osteoporosis (SOP) represents a significant health concern that impacts quality of life among the elderly population. Calcium supplementation is the most common treatment for those diagnosed with osteoporosis. However, the administration of inorganic calcium supplements is often associated with adverse effects, including constipation and nephrolithiasis. The fruit of Rosa roxburghii, commonly known as 'Ci Li' in Chinese, is both a medicine and a food. It aids in digestion, strengthens the spleen and stomach, and improves intestinal inflammation. The polysaccharides from R. roxburghii (RRFP) also exhibit activities such as enhancing immunity, managing diabetes, and regulating intestinal function. The current methods for RRFP extraction either have low extraction rates or are not conducive to scale-up applications; in addition, the effect of RRFP on improving SOP is unclear.

Results: RRFP with molecular weights ranging from 50 to 100 kDa was enriched by ultrafiltration, and was composed of fucose, rhamnose, arabinose, galactose, glucose, xylose, and galacturonic acid. The functional groups of polysaccharides were confirmed by Fourier transform infrared spectroscopy. Scanning electron microscopy revealed that RRFP exhibited a porous morphology. Notable increases in antioxidant capacity, dry femur weight, femur bone mineral density, and femur calcium levels were observed in RRFP group rats. Protein expression of keap1 decreased while HO-1 and Nrf2 increased after RRFP-Ca treatment. RRFP altered the composition of the intestinal microbiota in rats.

Conclusion: RRFP could enhance calcium absorption to improve SOP by activating the Nrf2/HO-1 pathway and modulating the gut microbiota, especially by altering the abundance of Lachnospiraceae. © 2026 Society of Chemical Industry.

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刺梨多糖的制备、表征及评价。水果对d-半乳糖和低钙饮食诱导的老年骨质疏松症的改善作用。

背景：老年骨质疏松症（SOP）是影响老年人生活质量的重要健康问题。补钙是骨质疏松症患者最常用的治疗方法。然而，无机钙补充剂的管理往往与不良反应，包括便秘和肾结石。刺梨（Rosa roxburghii）的果实，俗称“刺梨”，既是一种药物，也是一种食物。它有助于消化，健脾健胃，改善肠道炎症。刺梨多糖（RRFP）还具有增强免疫力、管理糖尿病和调节肠道功能等活性。目前提取rfp的方法要么提取率低，要么不利于规模化应用；此外，RRFP对SOP改进的作用尚不清楚。结果：经超滤富集得到分子量为50 ~ 100 kDa的RRFP，由焦糖、鼠李糖、阿拉伯糖、半乳糖、葡萄糖、木糖和半乳糖醛酸组成。用傅里叶红外光谱法确定了多糖的官能团。扫描电镜显示RRFP呈多孔形态。RRFP组大鼠抗氧化能力、股骨干重、股骨骨密度和股骨钙水平显著增加。经RRFP-Ca处理后，keap1蛋白表达降低，HO-1和Nrf2蛋白表达升高。RRFP改变了大鼠肠道菌群的组成。结论：RRFP可能通过激活Nrf2/HO-1通路，调节肠道菌群，特别是通过改变毛螺科菌的丰度，促进钙的吸收，从而改善SOP。©2026化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

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